Shanghai Tenth People's Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), School of Medicine, Tongji University, Shanghai, 200092, China.
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
J Nanobiotechnology. 2023 Nov 24;21(1):444. doi: 10.1186/s12951-023-02227-0.
Intestinal epithelia impairment of inflammatory bowel disease (IBD) leads to the leakage of bacteria and antigens and the consequent persistent immune imbalance. Restoring the epithelial barrier is a promising therapeutic target but lacks effective and safe clinical interventions. By identifying the catalase (CAT) presence in the IBD pathological environment, we herein develop a CAT-catalyzed pathologically coating on the damaged epithelial barrier to inhibit intestinal leakage for IBD therapy. With the codelivery of CaO (a CAT substrate) and dopamine, the nanosystem can enable CAT-catalyzed oxygen (O) production and in-situ polymerization of dopamine and then yield a thin and integrative polydopamine (PDA) coating on the intestinal barrier due to the highly adhesive property of PDA. In vivo study demonstrates that PDA coating provides not only a protective barrier by restricting intestinal leakage but also a favorable anti-inflammation effect. Beyond drug management, this work provides a physical repair strategy via catalyzed coating for IBD therapy.
肠上皮细胞损伤是炎症性肠病（IBD）的主要原因，会导致细菌和抗原的渗漏，进而引起持续的免疫失衡。修复上皮屏障是一种很有前途的治疗靶点，但目前缺乏有效和安全的临床干预措施。通过在 IBD 病理环境中鉴定出过氧化氢酶（CAT）的存在，我们在此开发了一种 CAT 催化的病理涂层，以抑制 IBD 治疗中的肠道渗漏。通过共递送 CaO（CAT 的底物）和多巴胺，该纳米系统能够实现 CAT 催化的氧气（O）生成和多巴胺的原位聚合，然后由于 PDA 的高粘附性，在肠屏障上生成一层薄且整合的聚多巴胺（PDA）涂层。体内研究表明，PDA 涂层不仅提供了一个限制肠道渗漏的保护屏障，而且还具有良好的抗炎作用。除了药物管理，这项工作还通过催化涂层为 IBD 治疗提供了一种物理修复策略。
