评价不同基质浓度和酶加载量下通过光发酵从小球藻中产生氢气的潜力。
Evaluation of hydrogen yield potential from Chlorella by photo-fermentation under diverse substrate concentration and enzyme loading.
机构信息
Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China; Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China.
Key Laboratory of New Materials and Facilities for Rural Renewable Energy, MOA of China, Henan Agricultural University, Zhengzhou 450002, China.
出版信息
Bioresour Technol. 2020 May;303:122956. doi: 10.1016/j.biortech.2020.122956. Epub 2020 Feb 4.
Chlorella is widely distributed, can be cultured in waste water and had short growth cycle. The high carbohydrate composition shows great potential for bioenergy output. In this work, concentrated Chlorella solution was adopted as raw material. Reducing sugar concentration, pH, and cumulative bio-hydrogen yield were taken as indexes, the effects of substrate concentration and enzyme (cellulase or neutral protease) load on photo-fermentation bio-hydrogen production process from microalgae biomass were investigated. Results showed that highest cumulative hydrogen yield was obtained at the optimal substrate concentration of 25 g/L, when the load of cellulase and protease are both 15%, the effect is the best which were 16.65 mL, 29.44 mL, and 43.62 mL, respectively. Results fitted well to the Gompertz model, indicating the feasibility of photo-fermentative bio-hydrogen production from concentrated Chlorella.
微藻分布广泛，可以在废水中培养且生长周期短。其富含碳水化合物，有很大的生物能源产出潜力。本工作以浓缩的小球藻溶液为原料，以还原糖浓度、pH 值和累计生物氢气产量为指标，考察了底物浓度和酶（纤维素酶或中性蛋白酶）负荷对微藻生物质光发酵生物制氢过程的影响。结果表明，在最佳底物浓度 25 g/L 下，可获得最高的累计氢气产量，当纤维素酶和蛋白酶的负载均为 15%时，效果最佳，分别为 16.65、29.44 和 43.62 mL。结果与 Gompertz 模型拟合较好，表明从浓缩小球藻中进行光合发酵生物制氢是可行的。
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