Zakharzhevskii Maxim, Drozdov Andrey S, Kolchanov Denis S, Shkodenko Liubov, Vinogradov Vladimir V
Laboratory of Solution Chemistry of Advanced Materials and Technologies, ITMO University, 197101 St. Petersburg, Russia.
Nanomaterials (Basel). 2020 Feb 12;10(2):313. doi: 10.3390/nano10020313.
Rapid detection of bacterial contamination is an essential task in numerous medical and technical processes and one of the most rapidly developing areas of nano-based analytics. Here, we present a simple-to-use and special-equipment-free test-system for bacteria detection based on magnetite nanoparticle arrays. The system is based on peroxide oxidation of chromogenic substrate catalyzed by magnetite nanoparticles, and the process undergoes computer-aided visual analysis. The nanoparticles used had a pristine surface free of adsorbed molecules and demonstrated high catalytic activities up to 6585 U/mg. The catalytic process showed the Michaelis-Menten kinetic with valued 1.22 mmol/L and V of 4.39 µmol/s. The nanoparticles synthesized were used for the creation of inkjet printing inks and the design of sensor arrays by soft lithography. The printed sensors require no special equipment for data reading and showed a linear response for the detection of model bacteria in the range of 10-10 colony-forming units (CFU) per milliliter with the detection limit of 3.2 × 10 CFU/mL.
快速检测细菌污染是众多医学和技术过程中的一项重要任务，也是基于纳米分析的发展最为迅速的领域之一。在此，我们展示了一种基于磁铁矿纳米颗粒阵列的、易于使用且无需特殊设备的细菌检测系统。该系统基于磁铁矿纳米颗粒催化的显色底物的过氧化物氧化反应，并且该过程经过计算机辅助视觉分析。所使用的纳米颗粒具有无吸附分子的原始表面，并表现出高达6585 U/mg的高催化活性。催化过程呈现米氏动力学， 值为1.22 mmol/L，V为4.39 µmol/s。合成的纳米颗粒用于制造喷墨打印墨水，并通过软光刻设计传感器阵列。打印的传感器无需特殊设备进行数据读取，并且在每毫升10 - 10菌落形成单位（CFU）的范围内对模型细菌的检测显示出线性响应，检测限为3.2×10 CFU/mL。
