Department of Pharmacy, Universidade Estadual de Maringá, Av. Colombo, 5790 Maringá PR, Brazil.
Food Departament, Universidade Tecnológica Federal de Campo Mourão, R. Rosalina Maria Ferreira, 1233 Vila Guaruja Campo Mourão, PR, Brazil.
J Mycol Med. 2020 Jun;30(2):100933. doi: 10.1016/j.mycmed.2020.100933. Epub 2020 Jan 24.
Essential oils (EO) are effective natural antimicrobials but are susceptible to oxidation. Microencapsulation improves EO stability, reduces toxicity, and controls release. The aim of this study was preparation, characterization and antidermatophytic activity of free and microencapsulated cinnamon essential oil (MP). MP were prepared by the spray drying method and the success of MP encapsulation was confirmed by UV-vis spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and Fourier transform infrared (FT-IR) spectroscopy. The antifungal effect of EO and MP was evaluated by the broth microdilution method against Microsporum gypseum and Trichophyton mentagrophytes. The checkerboard method was used to assess synergistic interactions. Fluorescence microscopy and scanning electron microscopy were used to investigate the inhibition of hyphal growth by EO and MP. A cytotoxic assay was performed using the VERO cell line. Microencapsulated cinnamon essential oil was found to be micrometric, with a round, regular structure. The minimum inhibitory concentration of EO was found to be between 125-250μg/mL, while that of MP was 220.5-440.5μg/mL. EO was synergistic with fluconazole while microencapsulated oil was less cytotoxic than EO.
精油（EO）是有效的天然抗菌剂，但容易氧化。微胶囊化可以提高 EO 的稳定性、降低毒性并控制释放。本研究的目的是制备、表征和评价游离和微胶囊化肉桂精油（MP）的抗皮肤真菌活性。MP 通过喷雾干燥法制备，并通过紫外可见光谱、动态光散射（DLS）、扫描电子显微镜（SEM）、差示扫描量热法（DSC）和傅里叶变换红外光谱（FT-IR）光谱来确认 MP 包封的成功。通过肉汤微量稀释法评估了 EO 和 MP 对石膏样小孢子菌和须癣毛癣菌的抗真菌作用。棋盘法用于评估协同作用。荧光显微镜和扫描电子显微镜用于研究 EO 和 MP 对菌丝生长的抑制作用。使用 VERO 细胞系进行细胞毒性测定。微胶囊化肉桂精油呈微米级，具有圆形、规则的结构。EO 的最小抑菌浓度（MIC）在 125-250μg/mL 之间，而 MP 的 MIC 在 220.5-440.5μg/mL 之间。EO 与氟康唑具有协同作用，而微胶囊化油的细胞毒性低于 EO。
