Department of Chemistry, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, 50-375, Wroclaw, Poland.
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, 50-375, Wroclaw, Poland.
Food Chem Toxicol. 2020 Apr;138:111183. doi: 10.1016/j.fct.2020.111183. Epub 2020 Feb 14.
Herbal formulations have been used in ethnomedicine and pharmacy around the world for thousands of years. One of them is Jerusalem Balsam (JB), which came into use in the seventeenth century. Today, people still produce and use it regularly as prophylactic supplement. JB has been widely used in Europe since the nineteenth century, as a remedy possessing antibacterial, antifungal and anti-inflammatory activities. The composition of the product was not known, although possible formulations were reported. In this study the original sample, which dated back to 1870, was investigated for chemical composition and cytotoxic activity. The obtained results were compared with results from more recently produced samples. Several tests were carried out, namely GC-MS, UPLC-PDA-Q-TOF-MS and MTT. Only the 150-year old sample showed a significant cytotoxic activity on cancer cell lines. At a concentration of 125 μg/mL after 72 h of incubation, the original sample inhibited almost 90% of cell metabolic activity, while contemporary samples showed none or little activity. None of the tested samples showed a significant impact on normal cells. These results may be attributed to the activities of benzoic acid and its derivatives, cinnamic acid derivatives, vanillin, group of sesquiterpenes and cembrene.
草药制剂在世界范围内的民族医学和药学中已经使用了数千年。其中之一是耶路撒冷香脂（JB），它在 17 世纪开始使用。如今，人们仍然定期生产和使用它作为预防性补充剂。自 19 世纪以来，JB 在欧洲被广泛使用，作为一种具有抗菌、抗真菌和抗炎活性的药物。尽管有报道称可能存在一些配方，但该产品的成分并不为人知。在这项研究中，对可追溯到 1870 年的原始样品进行了化学成分和细胞毒性研究。将获得的结果与最近生产的样品的结果进行了比较。进行了几项测试，即 GC-MS、UPLC-PDA-Q-TOF-MS 和 MTT。只有 150 年历史的样本在癌细胞系上显示出显著的细胞毒性。在孵育 72 小时后，浓度为 125μg/mL 时，原始样本几乎抑制了 90%的细胞代谢活性，而现代样本则没有或几乎没有活性。测试的样本均未对正常细胞产生显著影响。这些结果可能归因于苯甲酸及其衍生物、肉桂酸衍生物、香草醛、倍半萜类和石竹烯组的活性。
