British Columbia Centre for Disease Control, Provincial Health Services Authority, Vancouver, BC, Canada.
University of British Columbia, Vancouver, BC, Canada.
Influenza Other Respir Viruses. 2020 May;14(3):358-362. doi: 10.1111/irv.12722. Epub 2020 Feb 16.
Influenza viruses continually evolve to evade population immunity, and the different lineages are assigned into clades based on shared mutations. We have developed a publicly available computational workflow, the Influenza Classification Suite, for rapid clade mapping of sequenced influenza viruses. This suite provides a user-friendly workflow implemented in Galaxy to automate clade calling and antigenic site extraction. Workflow input includes clade definition and amino acid index array files, which can be customized to identify any clades of interest. The Influenza Classification Suite provides rapid, high-resolution understanding of circulating influenza strain evolution to inform influenza vaccine effectiveness and the need for potential vaccine reformulation.
流感病毒不断进化以逃避人群免疫，不同的谱系根据共同的突变被分配到进化枝中。我们开发了一个可供公众使用的计算工作流程，即流感分类套件，用于对测序流感病毒进行快速的进化枝映射。该套件提供了一个在 Galaxy 中实现的用户友好的工作流程，用于自动进行进化枝调用和抗原位点提取。工作流程输入包括进化枝定义和氨基酸指数数组文件，可自定义以识别任何感兴趣的进化枝。流感分类套件提供了对循环流感株进化的快速、高分辨率的了解，以告知流感疫苗的有效性和潜在疫苗改革的必要性。
