Rosenberg Jillian, Huang Jun
Committee on Cancer Biology, The University of Chicago.
Committee on Cancer Biology, The University of Chicago; Pritzker School of Molecular Engineering, The University of Chicago;
J Vis Exp. 2020 Jan 30(155). doi: 10.3791/59914.
The signaling and function of a cell are dictated by the dynamic structures and interactions of its surface receptors. To truly understand the structure-function relationship of these receptors in situ, we need to visualize and track them on the live cell surface with enough spatiotemporal resolution. Here we show how to use recently developed Lattice Light-Sheet Microscopy (LLSM) to image T-cell receptors (TCRs) four-dimensionally (4D, space and time) at the live cell membrane. T cells are one of the main effector cells of the adaptive immune system, and here we used T cells as an example to show that the signaling and function of these cells are driven by the dynamics and interactions of the TCRs. LLSM allows for 4D imaging with unprecedented spatiotemporal resolution. This microscopy technique therefore can be generally applied to a wide array of surface or intracellular molecules of different cells in biology.
细胞的信号传导和功能由其表面受体的动态结构和相互作用所决定。为了真正在原位理解这些受体的结构-功能关系，我们需要以足够的时空分辨率在活细胞表面可视化并追踪它们。在这里，我们展示了如何使用最近开发的晶格层光显微镜（LLSM）在活细胞膜上对T细胞受体（TCR）进行四维（4D，空间和时间）成像。T细胞是适应性免疫系统的主要效应细胞之一，在这里我们以T细胞为例表明这些细胞的信号传导和功能是由TCR的动力学和相互作用驱动的。LLSM允许以前所未有的时空分辨率进行4D成像。因此，这种显微镜技术通常可应用于生物学中不同细胞的各种表面或细胞内分子。
