College of Agronomy, Shihezi University, The Key Laboratory of Oasis Eco-agriculture, Xinjiang Production and Construction Group, Shihezi University, Shihezi 832003, China.
State Key Laboratory of Biology for Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Plant Dis. 2020 Apr;104(4):1231-1238. doi: 10.1094/PDIS-07-19-1347-RE. Epub 2020 Feb 15.
Powdery mildew, which is caused by f. sp. (), is a disease of wheat worldwide. Xiaobaidong is a Chinese wheat landrace, which still maintains good resistance against powdery mildew. To obtain more genetic markers closely linked to the powdery mildew resistance gene and narrow the candidate region for its isolation, new simple sequence repeats and cross intron-spanning markers were designed based on the genome sequence of cultivar Chinese Spring chromosome 7BL. The flanking markers and were found to be tightly linked to at genetic distances of 0.4 cM and 0.3 cM, respectively. The resistance locus was mapped to a 63.40 kb and 0.29 Mb region of the Chinese Spring genome and Zavitan genome, respectively. The linked markers of could be used as diagnostic markers for . The linked molecular markers and delineated genomic region in the sequenced Chinese Spring genome will assist the future map-based cloning of .
白粉病由 f. sp. ()引起，是一种世界性的小麦病害。小白冬是中国的一个小麦地方品种，它仍然对白粉病保持着良好的抗性。为了获得更多与白粉病抗性基因紧密连锁的遗传标记，缩小其分离的候选区域，基于 7BL 染色体中国春品种的基因组序列，设计了新的简单序列重复和跨内含子跨越标记。侧翼标记 和 分别与 遗传距离为 0.4 cM 和 0.3 cM 紧密连锁。抗性基因座被定位到中国春基因组和 Zavitan 基因组的 63.40 kb 和 0.29 Mb 区域。 与 的连锁标记可作为 的诊断标记。在测序的中国春基因组中与 连锁的分子标记和划定的基因组区域将有助于未来基于图谱的基因克隆。
