Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of PR China; State Key Laboratory Breeding Base of Marine Genetic Resources; Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2325-2333. doi: 10.1099/ijsem.0.004041. Epub 2020 Feb 17.
A taxonomic study was carried out of strain K7, which was isolated from deep-sea water collected from the Indian Ocean. The bacterium was Gram-stain-negative, aerobic, oxidase-negative, catalase-positive, rod-shaped and non-motile. Growth was observed at salinities of 0.5-10 % (optimum, 3 %), at a pH range of pH 6.0-10.0 (optimum, pH 7.0) and at temperatures of 10-40 °C (optimum, 28 °C). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K7 belonged to the family , with the high sequence similarities to the genera (92.2 %-94.4 %), (91.9 %-93.2 %), (92.1 %-92.6 %), (92.1 %-92.3 %), (91.9 %-92.1 %) and (91.8 %-92.1 %). The principal fatty acids were iso-C (28.4 %), iso-CG (14.2 %), summed feature 9 (iso-C ω and/or C 10-methyl; 11.6 %), iso-C 3-OH (10.0 %) and summed feature 3 (C 7 and/or C 6; 9.6 %). The G+C content of the chromosomal DNA was 35.8 mol%. The respiratory quinone was determined to be MK-6 (100 %). Phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipid and four unidentified lipids were detected. The combined genotypic and phenotypic data show that strain K7 represents a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed, with the type strain K7 (=MCCC 1A01093=KCTC 52325).
对从印度洋深海海水采集的菌株 K7 进行了分类学研究。该细菌革兰氏染色阴性，需氧，氧化酶阴性，过氧化氢酶阳性，呈杆状，无动力。在 0.5-10%的盐度（最佳 3%）、pH 值 6.0-10.0 范围（最佳 pH 值 7.0）和 10-40°C（最佳 28°C）的温度下观察到生长。基于 16S rRNA 基因序列的系统发育分析结果表明，菌株 K7 属于 科，与属的序列相似度较高，包括 （92.2%-94.4%）、 （91.9%-93.2%）、 （92.1%-92.6%）、 （92.1%-92.3%）、 （91.9%-92.1%）和 （91.8%-92.1%）。主要脂肪酸为 iso-C（28.4%）、iso-CG（14.2%）、summed feature 9（iso-C ω 和/或 C 10-methyl；11.6%）、iso-C 3-OH（10.0%）和 summed feature 3（C 7 和/或 C 6；9.6%）。染色体 DNA 的 G+C 含量为 35.8mol%。鉴定的呼吸醌为 MK-6（100%）。检测到磷脂酰乙醇胺、两种未鉴定的胺脂、两种未鉴定的磷脂和四种未鉴定的脂类。综合表型和基因型数据表明，菌株 K7 代表一个新属的新型种，提议建立一个新属，名为 属，新种为 ，其模式菌株为 K7（=MCCC 1A01093=KCTC 52325）。
