Chemistry Department, College of Natural and Applied Sciences , University of Dar es Salaam , P.O. Box 35061, Dar es Salaam , Tanzania.
Department of Chemistry and Molecular Biology , University of Gothenburg , SE-412 96 Gothenburg , Sweden.
J Nat Prod. 2020 Feb 28;83(2):316-322. doi: 10.1021/acs.jnatprod.9b00721. Epub 2020 Feb 18.
A new meroisoprenoid (), two heptenolides ( and ), two -benzylated flavonoids ( and ), and 11 known compounds (-) were isolated from leaf, stem bark, and root bark extracts of ssp. by chromatographic separation. The structures of the new metabolites - were established by NMR, IR, and UV spectroscopic and mass spectrometric data analysis. ()-Sphaerodiol (), ()-acetylmelodorinol (), 7-hydroxy-6-hydromelodienone (), and dichamanetin () inhibited the proliferation of (3D7, Dd2) with IC values of 1.4-10.5 μM, although these compounds also showed cytotoxicity against human embryonic kidney HEK-293 cells. None of the compounds exhibited significant disruption in protein translation when assayed in vitro.
从 ssp. 的叶、茎皮和根皮提取物中，通过色谱分离得到一个新的倍半萜 (), 两个庚烯醇内酯 ( 和 ), 两个 - 苄基化的类黄酮 ( 和 ), 以及 11 个已知化合物 (-)。新代谢产物 - 的结构通过 NMR、IR、UV 光谱和质谱数据分析确定。()-Sphaerodiol (), ()-acetylmelodorinol (), 7-hydroxy-6-hydromelodienone (), 和 dichamanetin () 抑制了疟原虫 (3D7, Dd2) 的增殖，IC 值为 1.4-10.5 μM，尽管这些化合物对人胚肾 HEK-293 细胞也表现出细胞毒性。在体外测定时，这些化合物均未显示出明显的蛋白质翻译中断。
