School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, P. R. China.
National Engineering Research Center of Seafood, Dalian 116034, P. R. China.
J Agric Food Chem. 2020 Mar 11;68(10):3163-3170. doi: 10.1021/acs.jafc.0c00242. Epub 2020 Mar 2.
The purpose of this work was to develop a facile strategy based on self-crosslinking between the core and wall materials in the coacervation system for effective procyanidins (PCs) encapsulation. The coacervates were constructed through the interaction of bioactive PCs, gelatin, and sodium alginate, followed by forming cationic bridge of sodium alginate-calcium ions to improve the stability of PCs. When the concentration of PCs and calcium ions were 6.25 and 0.24 mg/mL, respectively, the PC-loaded coacervates showed spherical shape with a size about 150 nm, and the microcapsulation efficiency and yield was 81.19 ± 1.47 and 87.86 ± 2.67%, respectively. The photothermal stability of PCs was effectively improved by embedding them in coacervates. The decrease of mitochondrial membrane potential in PC-12 cells induced by HO was significantly inhibited by PC coacervates, demonstrating an improved protection effect of PCs after being encapsulated in coacervates.
本工作旨在开发一种基于共凝聚体系中核与壁材之间自交联的简便策略，以实现有效包封原花青素（PCs）。共凝聚体是通过生物活性 PCs、明胶和海藻酸钠的相互作用构建的，然后通过海藻酸钠-钙离子形成阳离子桥来提高 PCs 的稳定性。当 PCs 和钙离子的浓度分别为 6.25 和 0.24mg/mL 时，负载 PCs 的共凝聚体呈约 150nm 的球形，微胶囊化效率和产率分别为 81.19±1.47%和 87.86±2.67%。将 PCs 包埋在共凝聚体中可有效提高其光热稳定性。HO 诱导 PC-12 细胞线粒体膜电位降低，PC 共凝聚体显著抑制其降低，表明 PCs 包埋在共凝聚体中后具有更好的保护作用。
