Department of Pharmacology, School of Basic Medical Science, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.
Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Antimicrob Agents Chemother. 2020 Apr 21;64(5). doi: 10.1128/AAC.02192-19.
is an important risk factor for gastric ulcers. However, antibacterial therapies increase the resistance rate and decrease the eradication rate of Inspired by the microaerophilic characteristics of , we aimed at effectively establishing an oxygen-enriched environment to eradicate and prevent the recurrence of The effect and the mechanism of an oxygen-enriched environment in eradicating and preventing the recurrence were explored and During oral administration and after drug withdrawal, counts were evaluated by Giemsa staining in animal cohorts. An oxygen-enriched environment in which could not survive was successfully established by adding hydrogen peroxide into several solutions and rabbit gastric juice. Hydrogen peroxide effectively killed in Columbia blood agar and special peptone broth. Minimum inhibition concentrations and minimum bactericidal concentrations of hydrogen peroxide were both relatively stable after promotion of resistance for 30 generations, indicating that hydrogen peroxide did not easily promote resistance in In models of Mongolian gerbils and Kunming mice, hydrogen peroxide has been shown to significantly eradicate and effectively prevent the recurrence of without toxicity and damage to the gastric mucosa. The mechanism of hydrogen peroxide causing death was related to the disruption of bacterial cell membranes. The oxygen-enriched environment achieved by hydrogen peroxide eradicates and prevents the recurrence of by damaging bacterial cell membranes. Hydrogen peroxide thus provides an attractive candidate for anti- treatment.
是胃溃瘍的重要危險因素。然而，抗菌治療會增加耐藥率，降低 根除率。受 微需氧特性的啟發，我們旨在有效建立富氧環境，以根除和預防 的復發。探討了富氧環境在根除和預防復發中的作用和機制。 在動物群中，通過吉姆薩染色評估口服和停藥後 的計數。通過向幾種溶液和兔胃汁中添加過氧化氫成功建立了 無法存活的富氧環境。過氧化氫在哥倫比亞血平板和特殊蛋白胨培養基中有效殺死 。在經過 30 代抗性誘導後，過氧化氫的最低抑制濃度和最低殺菌濃度均相對穩定，表明過氧化氫不易在 中誘發耐藥性。在蒙古沙鼠和昆明小鼠模型中，過氧化氫已被證明能顯著根除並有效預防 的復發，且對胃黏膜無毒性和損傷。過氧化氫導致 死亡的機制與細菌細胞膜的破壞有關。過氧化氫通過破壞細菌細胞膜來根除和預防 的復發。因此，過氧化氫為抗 治療提供了一個有吸引力的候選藥物。
