Peng Qin, Chen Yahui, Ding Li, Zhao Zimiao, Yan Peiyu, Storey Kenneth B, Shi Haitao, Hong Meiling
Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou, Hainan, China.
Department of Biology, Carleton University, Ottawa, Canada.
PeerJ. 2020 Feb 5;8:e8501. doi: 10.7717/peerj.8501. eCollection 2020.
During the early-life period, the hatchlings of red-eared slider turtles () rely on their own post-hatching internal yolk for several days before beginning to feed. The gut microbiome is critical for the adaptation of organisms to new environments, but, to date, how the microbiome taxa are assembled during early life of the turtle is unknown. In this study, the intestinal microbiome of red-eared slider hatchlings (fed on commercial particle food) was systematically analyzed at four different growth stages (0 d, 10 d, 20 d, 30 d) by a high-throughput sequencing approach. Results showed that the dominant phyla were Firmicutes (58.23%) and Proteobacteria (41.42%) at 0-day, Firmicutes (92.94%) at 10-day, Firmicutes (67.08%) and Bacteroidetes (27.17%) at 20-day, and Firmicutes (56.46%), Bacteroidetes (22.55%) and Proteobacteria (20.66%) at 30-day post-hatching. Members of the Bacteroidaceae family were absent in 0-day and 10-day turtles, but dominated in 20-day and 30-day turtles. The abundance of also showed the highest value in 10-day turtles. The richness of the intestinal microbiomes was lower at 0-day and 30-day than that at 10-day and 20-day, while the diversity was higher at 10-day and 30-day than that at 0-day and 20-day. The results endowed the turtles with an ability to enhance their tolerance to the environment.
在幼体期，红耳龟幼龟在开始进食前的几天依靠自身孵化后的内源性卵黄为生。肠道微生物群对生物体适应新环境至关重要，但迄今为止，龟类幼体早期微生物群分类群是如何组装的尚不清楚。在本研究中，采用高通量测序方法，在四个不同生长阶段（0天、10天、20天、30天）对红耳龟幼龟（喂食商业颗粒饲料）的肠道微生物群进行了系统分析。结果显示，孵化后0天时优势菌门为厚壁菌门（58.23%）和变形菌门（41.42%），10天时为厚壁菌门（92.94%），20天时为厚壁菌门（67.08%）和拟杆菌门（27.17%），30天时为厚壁菌门（56.46%）、拟杆菌门（22.55%）和变形菌门（20.66%）。拟杆菌科成员在0天和10天的龟中不存在，但在20天和30天的龟中占主导地位。[此处原文可能有缺失内容]的丰度在10天的龟中也显示出最高值。肠道微生物群的丰富度在0天和30天时低于10天和20天时，而多样性在10天和30天时高于0天和20天时。这些结果赋予了龟增强对环境耐受性的能力。
