Wang Yan, Wang Hanping, Cheng HuYin, Chang Fan, Wan Yi, She Xiaoping
College of Life Sciences, Shaanxi Normal University, Xi'an, China.
Shaanxi Microbiology Institute, Xi'an, China.
PeerJ. 2020 Feb 5;8:e8510. doi: 10.7717/peerj.8510. eCollection 2020.
The plant microbiome is one of the key determinants of plant health and metabolite production. The plant microbiome affects the plant's absorption of nutrient elements, improves plant tolerance to negative environmental factors, increases the accumulation of active components, and alters tissue texture. The microbial community is also important for the accumulation of secondary metabolites by plants. However, there are few studies on the niche differentiation of endophytic microorganisms of plants, especially at different elevations.
We investigated the effects of altitude on the community composition of endophytic fungal communities and the differentiation of endophytic microorganisms among different niches in Sm. The rhizosphere soil, roots, rhizomes and leaves of wild-type Sm. at different altitudes were sampled, and the fungal communities of all samples were analyzed by internal transcribed spacer one amplification sequencing.
The results showed that in rhizosphere soil, the number of operational taxonomic units (OTUs) that could be classified or identified decreased significantly with increasing altitude, whereas in the endosphere of plants, the total number of OTUs was higher at intermediate altitudes than other altitudes. Furthermore, the structural variability in the rhizosphere fungal community was significantly lower than that in the endophytic communities. In addition, our results confirmed the presence of niche differentiation among members of the endophytic microbial community. Finally, we also determined that the predominant genus of mycobiota in the rhizome was . This study provides insight into the relationships between the endosphere microbiome and plants and can guide the artificial cultivation of this plant.
植物微生物组是植物健康和代谢产物产生的关键决定因素之一。植物微生物组影响植物对营养元素的吸收，提高植物对负面环境因素的耐受性，增加活性成分的积累，并改变组织质地。微生物群落对于植物次生代谢产物的积累也很重要。然而，关于植物内生微生物的生态位分化研究较少，尤其是在不同海拔高度。
我们研究了海拔对内生真菌群落组成的影响以及内生微生物在不同生态位之间的分化情况。对不同海拔高度的野生型Sm.的根际土壤、根、根茎和叶进行采样，并通过内转录间隔区一扩增测序分析所有样品的真菌群落。
结果表明，在根际土壤中，可分类或鉴定的操作分类单元（OTU）数量随海拔升高而显著减少，而在植物内生菌中，OTU总数在中等海拔高度高于其他海拔高度。此外，根际真菌群落的结构变异性明显低于内生群落。此外，我们的结果证实了内生微生物群落成员之间存在生态位分化。最后，我们还确定根茎中真菌区系的优势属是。本研究为深入了解内生微生物组与植物之间的关系提供了见解，并可为该植物的人工栽培提供指导。
