Nannenga Brent L
Chemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, Arizona 85287, USA and Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, Arizona 85281, USA.
Struct Dyn. 2020 Feb 13;7(1):014304. doi: 10.1063/1.5128226. eCollection 2020 Jan.
Microcrystal electron diffraction, or MicroED, is a method that is capable of determining structure from very small and thin 3D crystals using a transmission electron microscope. MicroED has been successfully used on microcrystalline samples, including proteins, peptides, and small organic molecules, in many cases to very high resolutions. In this work, the MicroED workflow will be briefly described and areas of future method development will be highlighted. These areas include improvements in sample preparation, data collection, and structure determination.
微晶电子衍射（MicroED）是一种能够使用透射电子显微镜从非常小且薄的三维晶体中确定结构的方法。MicroED已成功应用于微晶样品，包括蛋白质、肽和小有机分子，在许多情况下都能达到非常高的分辨率。在这项工作中，将简要描述MicroED工作流程，并强调未来方法发展的领域。这些领域包括样品制备、数据收集和结构测定方面的改进。
