Aydemir Duygu, Simsek Gulsu, Ulusu Nuriye Nuray
Koc University, School of Medicine, Department of Medical Biochemistry, Sariyer, 34450, Istanbul, Turkey.
Koç University Research Center for Translational Medicine (KUTTAM), Sariyer, 34450, Istanbul, Turkey.
Data Brief. 2020 Feb 3;29:105218. doi: 10.1016/j.dib.2020.105218. eCollection 2020 Apr.
Minerals and trace elements play vital role in the biological functions for all organisms including human and other mammals. Therefore, imbalance in the mineral and/or trace element levels may cause formation of several diseases including cancer, diabetes, cardiovascular diseases, and neurological disorders. ICP-MS (inductively coupled plasma - mass spectrometry) is described as the most sensitive and accurate method. Here we reported an effective and fast protocol as method validation to evaluate trace element and minerals via ICP-MS in the mammalian tissue and serum samples. Our data showed that minimum relative standard deviation (RSD) values with the ICP-MS were observed when we used microwave digestion with the SUPRAPUR® grade nitric acid at the lower dilution rates. Our protocol validation may help researchers to measure trace elements and minerals in the mammalian samples fast, easily and accurately. EMSURE® grade HNO caused cross contamination in the serum and tissue samples. Our protocol validation may help researchers to measure trace elements and minerals in the mammalian samples fast, easily and accurately.
矿物质和微量元素在包括人类和其他哺乳动物在内的所有生物体的生物学功能中发挥着至关重要的作用。因此，矿物质和/或微量元素水平的失衡可能导致多种疾病的形成，包括癌症、糖尿病、心血管疾病和神经紊乱。电感耦合等离子体质谱法（ICP-MS）被认为是最灵敏和准确的方法。在此，我们报告了一种有效且快速的方案作为方法验证，用于通过ICP-MS评估哺乳动物组织和血清样本中的微量元素和矿物质。我们的数据表明，当我们在较低稀释率下使用SUPRAPUR®级硝酸进行微波消解时，观察到ICP-MS的最低相对标准偏差（RSD）值。我们的方案验证可能有助于研究人员快速、轻松且准确地测量哺乳动物样本中的微量元素和矿物质。EMSURE®级硝酸会在血清和组织样本中造成交叉污染。我们的方案验证可能有助于研究人员快速、轻松且准确地测量哺乳动物样本中的微量元素和矿物质。
