Badr Eman Ae, Assar Mohamed Fa, Eltorgoman Abdel Monem A, Labeeb Azza Zaghlol, Breaka Gehad A, Elkhouly Enas A
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine - Menoufia University, Egypt.
Biochemistry Division of Chemistry Department, Faculty of Science - Menoufia University, Egypt.
Biochem Biophys Rep. 2020 Feb 9;22:100747. doi: 10.1016/j.bbrep.2020.100747. eCollection 2020 Jul.
Accumulating evidence has revealed that livin gene and BCL-2 modifying factor (BMF) gene are closely associated with the initiation and progression of colon carcinoma by activating or suppressing multiple malignant processes. Those genes that can detect colon - cancer are a promising approach for cancer screening and diagnosis. This study aimed to evaluate correlation between livin, BMF and p53 genes expression in colon cancer tissues of patients included in the study, and their relationship with clinicopathological features and survival outcome in those patients. In this study, 50 pathologically diagnosed early cancer colon patients included and their tissue biopsy with 50 matched adjacent normal tissue, and 50 adenoma tissue specimens were analyzed for livin gene and BMF gene expressions using real time PCR. The relationship of those genes expressions with clinicopathological features, tumor markers, Time to Progression and overall survival for those patients were correlated in cancer colon group. In this study, there was a significant a reciprocal relationship between over expression of livin gene and down regulation of BMF and p53 genes in colon cancer cells. Livin mRNA was significantly higher, while BMF and p53 mRNA were significantly lower in colorectal cancer tissue compared to benign and normal colon tissue specimens (P < 0.001), however, this finding was absent between colon adenomas and normal mucosa. There was a significant association between up regulation of livin and down regulation of BMF and p53 expressions with more aggressive tumor (advanced TNM stage), rapid progression with metastasis and decreased overall survival in cancer colon patients, hence these genes can serve as significant prognostic markers of poor outcome in colon cancer patients. This work highlights the role of livin, BMF and p53 genes in colorectal tumorigenesis and the applicability of using those genes as a diagnostic and prognostic markers in patients with colon carcinoma and as a good target for cancer colon treatment in the future.
越来越多的证据表明，生存素基因和BCL-2修饰因子（BMF）基因通过激活或抑制多种恶性过程，与结肠癌的发生和发展密切相关。那些能够检测结肠癌的基因是癌症筛查和诊断的一种有前景的方法。本研究旨在评估生存素、BMF和p53基因在纳入研究的患者结肠癌组织中的表达相关性，以及它们与这些患者临床病理特征和生存结果的关系。在本研究中，纳入了50例经病理诊断的早期结肠癌患者及其组织活检样本，并与50份匹配的相邻正常组织以及50份腺瘤组织标本一起，使用实时PCR分析生存素基因和BMF基因的表达。在结肠癌组中，分析了这些基因表达与临床病理特征、肿瘤标志物、疾病进展时间和这些患者总生存的相关性。在本研究中，结肠癌细胞中生存素基因的过表达与BMF和p53基因的下调之间存在显著的相互关系。与良性和正常结肠组织标本相比，结直肠癌组织中生存素mRNA显著更高，而BMF和p53 mRNA显著更低（P < 0.001），然而，在结肠腺瘤和正常黏膜之间未发现此差异。生存素上调与BMF和p53表达下调之间存在显著关联，与更具侵袭性的肿瘤（晚期TNM分期）、伴有转移的快速进展以及结肠癌患者总生存降低相关，因此这些基因可作为结肠癌患者不良预后的重要预后标志物。这项工作突出了生存素、BMF和p53基因在结直肠癌发生中的作用，以及将这些基因用作结肠癌患者诊断和预后标志物的适用性，并且在未来可作为结肠癌治疗的良好靶点。
