Manisa Celal Bayar University, Art and Science Faculty, Department of Biology, Şehit Prof. Dr. İlhan Varank Campus, Manisa, Turkey.
Manisa Celal Bayar University, Art and Science Faculty, Department of Biology, Şehit Prof. Dr. İlhan Varank Campus, Manisa, Turkey.
Mar Pollut Bull. 2021 Nov;172:112750. doi: 10.1016/j.marpolbul.2021.112750. Epub 2021 Aug 10.
Hemocytes are the main immunologic parameters for invertebrate organisms as a response to diseases and pollutions. This study was aimed to reveal the relations between pollution and the total and differential hemocytes numbers. The gulf of İzmir was selected as location for sampling due to its contaminated by different anthropogenic pollutants mainly industrial effects. The effects of the pollutants were investigated on the mussels that have been collected from eight stations in the Izmir Bay (1- Inciralti, 2- Göztepe 3-Konak-4- Pasaport 5-Alsancak, 6-Karşıyaka, 7-Bostanlı), which are known as the most polluted part of inner Bay of Izmir (Western Coast of Turkey) and 8-Foça, (an anthropogenically industrialized and shipping -impacted area). The pollution effects in the bay were determined on hemolymphs by biomarkers such as total hemocyte counts (THCs) and differential hemocyte counts (DHCs). Microscopic examinations have shown the statistical differences at THCs and DHCs. The highest total hemocyte number was determined in the station 7 (Bostanlı) and the lowest in the station 4 (Pasaport) (P < 0.05). The number of total hemocyte counts were not differed significantly in the Stations among 2 (Göztepe), 5 (Alsancak) and 6 (Karşıyaka), and between Stations 8 (Foça) and 1 (Inciraltı) (P > 0.05). In addition, there have been significant differences in subpopulation according to differential hemocyte numbers. The results indicate that the station 8 (reference site-Foça) has the highest agranulocyte number while station 7 (Bostanlı) and the station 1 (İnciraltı) have the lowest agranulocyte numbers (P < 0.05). As for the basophilic hemocyte numbers, this situation was reversed highest number in station 1 (Inciraltı) and 7 (Bostanlı) and lowest in the station 8 (Foça) (p < 0.05). The station 4 (Pasaport) has the highest eosinophilic hemocyte number while station 8 (Foça) has the lowest. We concluded that differential hemocytes counts are more sensitive as biomarker with the highest numbers of agranulocytes and lowest numbers of eosinophilic and basophilic hemocytes in reference site.
血细胞是无脊椎动物对疾病和污染等反应的主要免疫参数。本研究旨在揭示污染与总血细胞和分类血细胞数量之间的关系。由于受到主要来自工业影响的各种人为污染物的污染，伊兹密尔湾被选为采样地点。在所采集的来自伊兹密尔湾八个站点（1-因克里尔蒂，2-戈兹泰佩，3-科纳克，4-帕萨波特，5-阿尔桑拉克，6-卡拉希萨卡，7-博斯丹利）的贻贝中研究了污染物的影响，这些贻贝被认为是伊兹密尔湾内湾（土耳其西海岸）最受污染的部分，8-福查（一个受到人为工业化和航运影响的地区）。通过总血细胞计数（THC）和分类血细胞计数（DHC）等生物标志物来确定海湾中的污染影响。显微镜检查表明，THC 和 DHC 存在统计学差异。在站 7（博斯丹利）中发现了最高的总血细胞数，而在站 4（帕萨波特）中发现了最低的总血细胞数（P<0.05）。站 2（戈兹泰佩）、站 5（阿尔桑拉克）和站 6（卡拉希萨卡）之间以及站 8（福查）和站 1（因克里尔蒂）之间的总血细胞计数在各站之间没有显著差异（P>0.05）。此外，根据分类血细胞数量，亚群之间存在显著差异。结果表明，站 8（参考站-福查）具有最高的无颗粒细胞数，而站 7（博斯丹利）和站 1（因克里尔蒂）具有最低的无颗粒细胞数（P<0.05）。对于嗜碱性血细胞数量，这种情况正好相反，站 1（因克里尔蒂）和站 7（博斯丹利）的数量最高，而站 8（福查）的数量最低（P<0.05）。站 4（帕萨波特）具有最高的嗜酸性粒细胞数，而站 8（福查）具有最低的嗜酸性粒细胞数。我们得出的结论是，与参考站点相比，分类血细胞计数作为生物标志物更敏感，因为它具有最高数量的无颗粒细胞和最低数量的嗜酸性粒细胞和嗜碱性粒细胞。
