Spüler M, Dimpfel W, Tüllner H U
Pro Science Private Research Institute, Linden, Fed. Rep. of Germany.
Arzneimittelforschung. 1988 Jul;38(7):881-4.
Streptozocin-diabetic rats were treated with a mixture of gangliosides (Cronassial, 21% GM1, 40%GD1a, 16% GD1b, 19% GT1b) in different application schemes: a) daily s.c. injection of 20 mg/kg gangliosides starting from the first day of diabetes and continued for 8 weeks; b) application of the same dose but only every second day over the same period of time; c) daily application of the same dose is started four weeks after induction of diabetes and continued for 4 weeks; d) daily injections of a corresponding volume of physiological saline. Serial in vivo determinations of conduction velocities in the tail nerve of these animals and of non-diabetic control animals gave the following results: 1. The nerve conduction velocities decreased in all diabetic groups which is evidence for the development of a peripheral diabetic neuropathy. 2. The finding from a previous study is reproduced showing that daily application of gangliosides counteracts this deceleration of nerve conduction. 3. Application of the same dose every second day is nearly as effective in inhibiting the development of the diabetic neuropathy as daily injections. 4. When a daily ganglioside treatment is started four weeks after the onset of diabetes, the existing neuropathy is reduced. The possible basis for this ganglioside effect on experimental peripheral neuropathy and its significance for the treatment of human diabetic polyneuropathies is discussed.
将链脲佐菌素诱导的糖尿病大鼠按照不同的给药方案用神经节苷脂混合物（Cronassial，21%GM1、40%GD1a、16%GD1b、19%GT1b）进行治疗：a）从糖尿病发病第一天开始，每日皮下注射20mg/kg神经节苷脂，持续8周；b）在相同时间段内，每隔一天给予相同剂量；c）在糖尿病诱导四周后开始每日给予相同剂量，并持续4周；d）每日注射相应体积的生理盐水。对这些动物以及非糖尿病对照动物的尾神经传导速度进行系列体内测定，结果如下：1. 所有糖尿病组的神经传导速度均降低，这证明发生了周围性糖尿病神经病变。2. 重复了先前研究的结果，即每日应用神经节苷脂可抵消神经传导的这种减慢。3. 每隔一天给予相同剂量在抑制糖尿病神经病变发展方面的效果与每日注射几乎相同。4. 在糖尿病发病四周后开始每日给予神经节苷脂治疗，现有的神经病变会减轻。讨论了这种神经节苷脂对实验性周围神经病变作用的可能机制及其对人类糖尿病性多发性神经病变治疗的意义。
