Spüler M, Dimpfel W, Tüllner H U
Arch Int Pharmacodyn Ther. 1987 Jun;287(2):211-23.
Diabetic neuropathy was induced in male inbred rats by a single injection of streptozotocin. The diabetic state of the animals was prevented from detoriation by daily injections of small amounts of insulin. Before as well as 4 and 8 weeks after induction of diabetes compound action potentials evoked by electrical stimulation were repeatedly recorded from muscle and tail nerves of the same 55 animals. The motor nerve as well as the maximal proximal and distal nerve conduction velocity were determined. The neuropathy was established by comparing the increase in conduction velocity in a nondiabetic control group with the marked decrease in a group of diabetic animals receiving daily placebo injections. In a second group of diabetic animals daily injections of gangliosides (Cronassial) significantly counteracted the slowing of nerve conduction in comparison to that observed in the diabetic placebo group during the development of the neuropathy. This effect is discussed in terms of an increase of Na+-K+-ATPase or neuronal sprouting, possibly induced by the daily ganglioside application, and its usefulness in ameliorating human diabetic neuropathy.
通过单次注射链脲佐菌素在雄性近交系大鼠中诱导糖尿病性神经病变。通过每日注射少量胰岛素防止动物的糖尿病状态恶化。在糖尿病诱导前以及诱导后4周和8周，对同一55只动物的肌肉和尾神经反复记录电刺激诱发的复合动作电位。测定运动神经以及最大近端和远端神经传导速度。通过比较非糖尿病对照组传导速度的增加与每日接受安慰剂注射的糖尿病动物组中传导速度的显著降低来确定神经病变。在第二组糖尿病动物中，与糖尿病安慰剂组在神经病变发展过程中观察到的情况相比，每日注射神经节苷脂（Cronassial）显著抵消了神经传导的减慢。根据每日应用神经节苷脂可能诱导的Na+-K+-ATP酶增加或神经元发芽及其在改善人类糖尿病性神经病变中的作用来讨论这种效应。
