Knobbe Tim J, Douwes Rianne M, Kremer Daan, Swarte J Casper, Eisenga Michele F, Gomes-Neto António W, van Londen Marco, Peters Frans T M, Blokzijl Hans, Nolte Ilja M, Hendriks Wouter H, Harmsen Hermie J M, Bakker Stephan J L
Department of Internal Medicine, Division of Nephrology, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands.
Department of Gastroenterology and Hepatology, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands.
J Clin Med. 2020 Feb 14;9(2):518. doi: 10.3390/jcm9020518.
Renal transplant recipients (RTRs) often suffer from posttransplant diarrhea. The observed dysbiosis in RTR may influence the fermentation processes in the gut. In this study, we aimed to investigate whether fermentation differs between RTRs and healthy controls (HCs), by measuring breath H and CH concentrations. Additionally, we determined the fecal presence of the methanogen (), which plays a main role in the process of methanogenesis. Data from the TransplantLines Biobank and Cohort Study (NCT03272841) was used. A total of 142 RTRs and 77 HCs were included. Breath H concentrations in RTRs were not significantly different from HCs. Breath CH concentrations in RTRs were significantly lower compared with HCs (median [interquartile range (IQR)] 7.5 [3.9-10.6] ppm vs. 16.0 [8.0-45.5] ppm, < 0.001). was less frequently present in the feces of RTRs compared to HCs (28.6% vs. 86.4% resp., < 0.001). Our findings regarding the altered methanogenesis in the gut of RTRs show similarities with previous results in inflammatory bowel disease patients. These findings provide novel insight into the alterations of fermentation after renal transplantation, which may contribute to understanding the occurrence of posttransplant diarrhea.
肾移植受者（RTRs）常患有移植后腹泻。在RTRs中观察到的微生物群失调可能会影响肠道中的发酵过程。在本研究中，我们旨在通过测量呼出气体中氢气（H）和甲烷（CH）的浓度，调查RTRs与健康对照者（HCs）之间的发酵情况是否存在差异。此外，我们还测定了产甲烷菌（）在粪便中的存在情况，其在甲烷生成过程中起主要作用。使用了来自TransplantLines生物样本库和队列研究（NCT03272841）的数据。共纳入了142名RTRs和77名HCs。RTRs呼出气体中的H浓度与HCs无显著差异。与HCs相比，RTRs呼出气体中的CH浓度显著更低（中位数[四分位间距（IQR）]为7.5 [3.9 - 10.6] ppm，而HCs为16.0 [8.0 - 45.5] ppm，< 0.001）。与HCs相比，RTRs粪便中产甲烷菌的存在频率更低（分别为28.6%和86.4%，< 0.001）。我们关于RTRs肠道中甲烷生成改变的研究结果与先前炎症性肠病患者的结果相似。这些发现为肾移植后发酵的改变提供了新的见解，这可能有助于理解移植后腹泻的发生。
