Laboratory of Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Leuven, Belgium.
Chem Commun (Camb). 2020 Mar 17;56(22):3317-3320. doi: 10.1039/c9cc08938a.
In this work, the preparation of new S-adenosyl-l-methionine (SAM) analogues for sequence specific DNA labeling is evaluated. These non-natural analogues, comprising cysteine rather than the natural homolog, were obtained in near quantitative conversions from readily available starting materials without relying on using an excess amount of labor intensive molecules. The synthetic strategy was used to generate fluorescent cofactors, with colours spanning the whole visible spectrum, and their applicability in methyltransferase based optical mapping is shown.
在这项工作中，评估了新的 S-腺苷-L-甲硫氨酸（SAM）类似物用于序列特异性 DNA 标记的制备。这些非天然类似物由半胱氨酸而不是天然同系物组成，可从易得的起始原料中以接近定量的转化率获得，而无需依赖使用过量的劳动密集型分子。该合成策略用于生成荧光辅因子，其颜色涵盖整个可见光谱，并展示了它们在基于甲基转移酶的光学作图中的适用性。
