Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
Food Chem. 2020 Jul 1;317:126229. doi: 10.1016/j.foodchem.2020.126229. Epub 2020 Jan 25.
The impact of nanoemulsions containing triglycerides with different fatty acid chain lengths on the bioavailability of a highly lipophilic bioactive: 5-demethylnobiletin (5-DN) was investigated. 5-DN was encapsulated in nanoemulsions fabricated using either medium-chain triglycerides (MCT) or long-chain triglycerides (LCT). They were then subjected to in vitro digestion, and the resulting mixed micelles was applied to a Caco-2 cell model. Higher 5-DN bioaccessibility was found for the MCT-nanoemulsion (13%) than for the LCT-nanoemulsion (7%). However, only 30% 5-DN in MCT crossed the Caco-2 monolayer and 50% was metabolized, while 60% 5-DN in LCT crossed the monolayer and only 10% was metabolized. More lipid droplets and chylomicrons were also formed for the LCT nanoemulsions, indicating greater 5-DN transported through lymph. Although MCT gave a higher 5-DN bioaccessibility, the final amount of 5-DN absorbed and transported to the lymph was inferior to that of the LCT formulation.
研究了不同脂肪酸链长的甘油三酯纳米乳对高度亲脂性生物活性物质 5-去甲川陈皮素（5-DN）生物利用度的影响。将 5-DN 包封在使用中链甘油三酯（MCT）或长链甘油三酯（LCT）制备的纳米乳液中。然后对其进行体外消化，并将得到的混合胶束应用于 Caco-2 细胞模型。MCT-纳米乳液（13%）的 5-DN 生物利用度高于 LCT-纳米乳液（7%）。然而，只有 30%的 MCT 中的 5-DN 穿过 Caco-2 单层，且 50%被代谢，而 60%的 LCT 中的 5-DN 穿过单层，且只有 10%被代谢。LCT 纳米乳液还形成了更多的脂质滴和乳糜微粒，表明更多的 5-DN 通过淋巴转运。尽管 MCT 使 5-DN 的生物利用度更高，但吸收和转运到淋巴的 5-DN 的最终量低于 LCT 制剂。
