Kilpatrick Laurie E, Kiani Mohammad F
Center for Inflammation, Translational and Clinical Lung Research, Department of Thoracic Medicine and Surgery, Lewis Katz School of Medicine at Temple University, Philadelphia, Pennsylvania.
Department of Mechanical Engineering, College of Engineering, Temple University, Philadelphia, Pennsylvania.
Shock. 2020 May;53(5):585-595. doi: 10.1097/SHK.0000000000001407.
Sepsis is a life-threatening syndrome of organ dysfunction caused by a dysregulated host response to infection characterized by excessive neutrophil infiltration into vital organs. In sepsis, patients often die of organ failure and therapies directed against endothelial cell dysfunction and tissue damage are important targets for treatment of this disease. Novel approaches are required to understand the underlying pathophysiology of neutrophil dysregulation and neutrophil-endothelial cell interactions that play a critical role in the early course of organ damage and disruption of endothelial protective barrier. Here, we review methodologies that our laboratories have employed to study neutrophil-endothelial interaction and endothelial barrier function in in vivo and in vitro models of sepsis. We will focus on in vivo rodent models of sepsis and in vitro tools that use human cell culture models under static conditions and the more physiologically relevant biomimetic microfluidic assays. This Methods paper is based on our presentation in the Master Class Symposium at the 41st Annual Conference on Shock 2018.
脓毒症是一种由宿主对感染的失调反应引起的危及生命的器官功能障碍综合征，其特征是中性粒细胞过度浸润到重要器官。在脓毒症中，患者常死于器官衰竭，针对内皮细胞功能障碍和组织损伤的治疗是该疾病治疗的重要靶点。需要新的方法来理解中性粒细胞失调以及中性粒细胞与内皮细胞相互作用的潜在病理生理学，这些相互作用在器官损伤和内皮保护屏障破坏的早期过程中起关键作用。在此，我们综述了我们实验室用于在脓毒症的体内和体外模型中研究中性粒细胞与内皮细胞相互作用以及内皮屏障功能的方法。我们将重点关注脓毒症的体内啮齿动物模型以及在静态条件下使用人类细胞培养模型的体外工具，以及更具生理相关性的仿生微流控分析方法。本方法学论文基于我们在2018年第41届休克年会主课堂研讨会上的报告。
