Siddik Muhammad A B, Fotedar Ravi, Chaklader Md Reaz, Foysal Md Javed, Nahar Ashfaqun, Howieson Janet
Department of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Patuakhali, Bangladesh.
School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.
Front Physiol. 2020 Jan 31;10:1635. doi: 10.3389/fphys.2019.01635. eCollection 2019.
A feeding trial was carried out to evaluate the effects of substitution of fishmeal (FM) by dietary poultry by-product meal, fermented by and on growth, intestinal health, microbial composition, immune related cytokines and disease resistance of freshwater crayfish, marron () against Two isonitrogenous and isocaloric diets were formulated by replacing FM protein with fermented poultry by-product meal (FPBM) protein at 0% (Control) and 75% (FPBM), and fed marron for 70 days. The results indicated no significant difference ( > 0.05) in final body weights between two groups of marron, whilst intestinal microvilli number per fold was increased in marron fed FPBM than the control. The 16S rRNA sequences revealed an increased number of and , and decreased number of at genus level in the distal intestine of marron fed FPBM. Marron fed FPBM showed up-regulated expression of IL-8, IL-10, and IL-17F genes in the distal intestine. Significantly ( < 0.05) increased lysozyme and phagocytic activity, and higher survival was found in marron fed FPBM following a bacterial challenge with . Therefore, it is concluded that FPBM is beneficial to marron in terms of microbial community, immune-related cytokines and disease resistance against
进行了一项饲养试验，以评估用由[具体微生物1]和[具体微生物2]发酵的饲用家禽副产品粉替代鱼粉（FM）对淡水小龙虾马龙（Cherax tenuimanus）生长、肠道健康、微生物组成、免疫相关细胞因子及抗病力的影响。以0%（对照）和75%（发酵家禽副产品粉，FPBM）的比例用发酵家禽副产品粉（FPBM）蛋白替代FM蛋白，配制了两种等氮等热量的饲料，并投喂马龙70天。结果表明，两组马龙的终末体重无显著差异（P>0.05），而投喂FPBM的马龙每褶皱的肠道微绒毛数量比对照组增加。16S rRNA序列显示，投喂FPBM的马龙远端肠道中，在属水平上[有益菌属1]和[有益菌属2]的数量增加，[有害菌属]的数量减少。投喂FPBM的马龙远端肠道中IL-8、IL-10和IL-17F基因的表达上调。在用[病原菌名称]进行细菌攻毒后，投喂FPBM的马龙溶菌酶和吞噬活性显著（P<0.05）提高，存活率更高。因此，得出结论，就微生物群落、免疫相关细胞因子及对[病原菌名称]的抗病力而言，FPBM对马龙有益。
