School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh.
School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.
Fish Shellfish Immunol. 2021 Jan;108:42-52. doi: 10.1016/j.fsi.2020.11.020. Epub 2020 Nov 21.
Probiotic supplements are being used to improve the growth and immune performance of aquaculture species over the last couple of decades. In recent times, black soldier fly (BSF) is considered as one of the promising sources of alternative protein to fishmeal protein in aqua-diets. Since the freshwater crayfish, marron (Cherax cainii), a Western Australian's native and iconic freshwater crayfish species, grows fairly slow under commercial farming environment, this study was aimed to investigate the supplemental effect of BSF and BSF with probiotic bacteria Lactobacillus plantarum (BSFLP) on overall health and immune performance of marron after 56 days of feeding under laboratory conditions. The post-trial data revealed insignificant influences of any diets on growth performance, however, both BSF and BSFLP based diets significantly improved some haemolymph parameters and gut health of marron. High throughput sequence data revealed that both BSF and BSFLP diets significantly improved the diversity of microbial communities including some beneficial bacteria for crustaceans in the hindgut of marron. Further analysis showed that both BSF and BSFLP diets upregulated the expression of some genes in the gut tissue and haemocytes associated with the innate immune response of marron at 48 h post injection. The up-regulation of some immune genes in BSFLP diet group was found significantly linked to OTU abundance for Lactobacillus. The findings of this study could be helpful for improving overall health status of marron.
在过去的几十年中，益生菌补充剂被用于提高水产养殖物种的生长和免疫性能。最近，黑水虻（BSF）被认为是替代水产饲料中鱼粉蛋白的有前途的蛋白质来源之一。由于澳大利亚西部特有的淡水螯虾，马龙螯虾（Cherax cainii）在商业养殖环境下生长相当缓慢，因此本研究旨在研究 BSFL 和添加益生菌植物乳杆菌（BSFLP）对马龙在实验室条件下饲养 56 天后的整体健康和免疫性能的补充作用。试验后数据显示，任何饮食对生长性能均无显著影响，但 BSFL 和 BSFLP 饮食均显著改善了马龙的一些血淋巴参数和肠道健康。高通量测序数据显示，BSFL 和 BSFLP 饮食均显著改善了马龙后肠中包括一些对甲壳类动物有益的微生物群落的多样性。进一步分析表明，BSFL 和 BSFLP 饮食在注射后 48 小时上调了与马龙先天免疫反应相关的肠道组织和血细胞中一些基因的表达。BSFLP 饮食组中一些免疫基因的上调与乳杆菌的 OTU 丰度显著相关。本研究的结果可能有助于改善马龙的整体健康状况。
