Dao Thi Thanh Thuy, Fotedar Ravi
School of Molecular and Life Sciences, Curtin University, 1 Turner Avenue, Bentley, WA 6102, Australia.
Research Institute for Aquaculture No. 3 (RIA3), 02 Dang Tat Street, Nha Trang 650000, Vietnam.
Animals (Basel). 2024 Dec 12;14(24):3591. doi: 10.3390/ani14243591.
We investigated the effect of feed deprivation for 45 days on the growth, immunity, and health of 0marron () initially fed for 110 days on various protein sources including fishmeal (FM), poultry by-product meal (PBM), black soldier fly meal (BSFM), soybean meal (SBM), lupin meal (LM), and tuna hydrolysate. The marron were weighed and sacrificed immediately after feeding stopped (day 0) and at days 15, 30, and 45 after the feed deprivation trial commenced. Total haemolymph count, differential haemocyte count, lysozyme activity, protease activity, total bacterial count in the digestive tract, and organosomatic indices were analysed. Initially feeding marron any protein sources did not influence the percentage of weight gain and specific growth rates of marron. All marron showed more than 83% survival; however, marron fed soybean meal showed significantly lower survival than others. Dietary sources of protein altered organosomatic indices of starved marron during various starvation periods and resulted in a significant decrease in total haemocyte counts, lysozyme activity, protease activity, and bacterial count in the digestive tract of marron. Starved marron initially fed PBM and BSFM showed higher tolerance to starvation, followed by marron initially fed FM and SBM, while marron initially fed TH and LM showed the highest susceptibility to starvation.
我们研究了对初始投喂110天、以包括鱼粉（FM）、家禽副产品粉（PBM）、黑水虻粉（BSFM）、豆粕（SBM）、羽扇豆粉（LM）和金枪鱼水解物在内的各种蛋白质来源喂养的墨瑞小龙虾（ ）进行45天饥饿处理，对其生长、免疫力和健康状况的影响。在停止投喂后（第0天）以及饥饿试验开始后的第15、30和45天，对墨瑞小龙虾进行称重并立即宰杀。分析了总血淋巴计数、血细胞分类计数、溶菌酶活性、蛋白酶活性、消化道中的总细菌计数以及脏体指数。最初用任何一种蛋白质来源投喂墨瑞小龙虾，均不影响其体重增加百分比和特定生长率。所有墨瑞小龙虾的存活率均超过83%；然而，用豆粕喂养的墨瑞小龙虾的存活率显著低于其他组。在不同饥饿时期，蛋白质的饮食来源改变了饥饿墨瑞小龙虾的脏体指数，并导致墨瑞小龙虾消化道中的总血细胞计数、溶菌酶活性、蛋白酶活性和细菌计数显著下降。最初用PBM和BSFM喂养的饥饿墨瑞小龙虾对饥饿的耐受性较高，其次是最初用FM和SBM喂养的墨瑞小龙虾，而最初用TH和LM喂养的墨瑞小龙虾对饥饿的易感性最高。
