School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh.
Environ Microbiol Rep. 2022 Apr;14(2):286-298. doi: 10.1111/1758-2229.13049. Epub 2022 Feb 7.
To reduce the reliance on fishmeal (FM), other protein sources have been evaluated on cultured animals. In a 60-days feeding trial, marrons (Cherax cainii) were fed a FM diet and five test diets containing 100% of plant-based protein sources such as soybean, lupin and valorised animal-based proteins such as poultry-by-product, black soldier fly and tuna hydrolysate. At the end of the trial, DNA samples from marron gut and rearing water were investigated through DNA-based 16S rRNA gene sequencing. Plant-based diets increased abundance for Aeromonas, Flavobacterium and Vogesella, whereas animal and insect proteins influenced diverse bacterial groups in the gut linked to various metabolic activities. Insect meal in the water favoured the growth of Firmicutes and lactic acid bacteria, beneficial for the marron health. Aeromonas richness in the gut and reared water signified the ubiquitous nature of the genus in the environment. The higher bacterial diversity in the gut and water with PBP and BSF was further supported by qPCR quantification of the bacterial single-copy gene, rpoB. The overall results suggested that PBP and BSF can exhibit positive and influential effects on the gut and water microbial communities, hence can be used as sustainable ingredients for the crayfish aquaculture.
为了减少对鱼粉（FM）的依赖，人们已经对其他蛋白质来源进行了评估，以用于养殖动物。在为期 60 天的饲养试验中，用 FM 饲料和五种含有 100%植物性蛋白质来源（如大豆、羽扇豆和增值动物蛋白质来源）的试验饲料来喂养淡水小龙虾。在试验结束时，通过基于 DNA 的 16S rRNA 基因测序，研究了淡水小龙虾肠道和养殖水的 DNA 样本。植物性饲料增加了气单胞菌、黄杆菌和沃氏菌的丰度，而动物和昆虫蛋白则影响了肠道中与各种代谢活动相关的多种细菌群。水中的昆虫饲料有利于厚壁菌门和乳酸菌的生长，这对淡水小龙虾的健康有益。肠道和养殖水中的气单胞菌丰富度表明该属在环境中普遍存在。用 PBP 和 BSF 处理时，肠道和水中的细菌多样性更高，这进一步得到了细菌单拷贝基因 rpoB 的 qPCR 定量的支持。总体结果表明，PBP 和 BSF 可以对肠道和水微生物群落产生积极和有影响的作用，因此可以用作小龙虾水产养殖的可持续成分。
