Department of AIDS Prevention and Control, Shaanxi Provincial Center for Disease Control and Prevention, Xi'an, China.
Department of AIDS Prevention and Control, Shaanxi Provincial Center for Disease Control and Prevention, Xi'an, China.
Infect Genet Evol. 2020 Jul;81:104257. doi: 10.1016/j.meegid.2020.104257. Epub 2020 Feb 19.
HIV-1 genetic distribution and recombinant patterns are important in understanding the HIV epidemic among men who have sex with men (MSM). In this study, 83 HIV-positive MSM infections were confirmed at a sentinel surveillance site in Xi'an city, China in 2018. HIV-1 genotypes were determined by phylogenetic analyses of HIV-1 gag, pol and env gene fragments, including CRF07_BC (51.8%), CRF01_AE (30.1%), subtype B (3.6%), CRF55_01B (3.6%), CRF104_0107 (1.2%) and unique recombinant forms (URFs) (9.6%). Transmitted drug resistance mutations were detected in 2.4% (2/82) of HIV-infected MSM individuals. The phylogenetic analyses of near full-length genome (NFLG) of HIV-1 URFs were performed. A new circulating recombinant form (CRF), designated as CRF104_0107, was found in three epidemiologically unlinked individuals in Shaanxi province, China. The CRF104_0107 is composed of genomes CRF01_AE and CRF07_BC, with six recombinant breakpoints in the gag, pol, vif and vpr genes. This second-generation CRF has a breakpoint (HXB2 nt 3011) in common with CRF07_BC. The emergence of novel CRF and multiple URFs reflected HIV-1 genetic complexity among the local key populations in Xi'an city, China.
在中国西安市的一个哨点监测点，2018 年共确认了 83 例感染 HIV-1 的男男性行为者（MSM）。通过对 HIV-1 gag、pol 和 env 基因片段的系统进化分析，确定了 HIV-1 基因型，包括 CRF07_BC（51.8%）、CRF01_AE（30.1%）、B 亚型（3.6%）、CRF55_01B（3.6%）、CRF104_0107（1.2%）和独特重组形式（URFs）（9.6%）。在 2.4%（2/82）的 HIV 感染 MSM 个体中检测到传播耐药突变。对 HIV-1 URF 全长基因（NFLG）的系统进化分析表明，在中国陕西省的 3 个无关联的个体中发现了一种新的循环重组形式（CRF），被命名为 CRF104_0107。该 CRF104_0107 由 CRF01_AE 和 CRF07_BC 基因组组成，在 gag、pol、vif 和 vpr 基因中有 6 个重组断点。该第二代 CRF 与 CRF07_BC 有一个共同的断点（HXB2 nt 3011）。新型 CRF 和多种 URF 的出现反映了中国西安市当地关键人群中 HIV-1 遗传的复杂性。
