He Bing, Shi Pibiao, Lv Yuanda, Gao Zhiping, Chen Guoxiang
School of Life Sciences, Nanjing Normal University, Nanjing 210023, Jiangsu, People's Republic of China.
J Genet. 2020;99.
Shi-related sequence (SRS) proteins are plant-specific transcription factors that play important roles in developmental processes, including regulating hormone biosynthesis, response or signal transduction. However, systematical analysis of the gene family in maize has not yet been conducted. In this study, 11 genes with 13 transcripts were identified and characterized. The characteristics of the gene family were analysed in terms of phylogenetic relationships, chromosome distribution and gene structure. RNA-sequencing data analysis showed that the expression patterns of genes were quite different from each other in maize, indicating their divergence in function. Interestingly, the gene is highly expressed in senescent leaves. Using further coexpression network analysis, we determined that the module containing were over-represented by genes related to abscisic acid (ABA) stimulus and carbohydrate metabolic process. This result indicated that might perceive ABA signal and cause the activation of carbohydrate remobilization during leaf ageing. This study provides valuable information for understanding the functions of the SRS genes in maize.
Shi相关序列（SRS）蛋白是植物特有的转录因子，在发育过程中发挥重要作用，包括调节激素生物合成、响应或信号转导。然而，尚未对玉米中的该基因家族进行系统分析。在本研究中，鉴定并表征了11个基因及其13个转录本。从系统发育关系、染色体分布和基因结构方面分析了该基因家族的特征。RNA测序数据分析表明，玉米中各基因的表达模式差异很大，表明它们在功能上存在差异。有趣的是，该基因在衰老叶片中高度表达。通过进一步的共表达网络分析，我们确定包含该基因的模块在与脱落酸（ABA）刺激和碳水化合物代谢过程相关的基因中过度富集。这一结果表明，该基因可能感知ABA信号并导致叶片衰老过程中碳水化合物再动员的激活。本研究为理解玉米中SRS基因的功能提供了有价值的信息。
