CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, No.11, Beiyitiao, Zhongguancun, Beijing, 100190, P. R. China.
School of Nanoscience and Technology, University of Chinese Academy of Sciences, No. 19(A) Yuquan Rd, Shijingshan District, Beijing, 100049, P. R. China.
Adv Mater. 2020 Apr;32(14):e1907718. doi: 10.1002/adma.201907718. Epub 2020 Feb 24.
To explore highly sensitive and low-toxicity techniques for tracking and evaluation of non-small-cell lung cancer (NSCLC), one of the most mortal tumors in the world, it is utterly imperative for doctors to select the appropriate treatment strategies. Herein, developing near-infrared (NIR) excited nanosensors, in which the donor and acceptor pairs within a biological metal-organic framework (bio-MOF) matrix are precisely controlled to rationalize upconversion Förster resonance energy transfer (FRET), is suggested for detecting the O concentration inside tumors with reduced signal disturbance and health detriment. Under NIR excitation, as-fabricated core/satellite nanosensors exhibit much improved FRET efficiency and reversible hypoxic response with high sensitivity, which are effective both in vitro and in vivo (zebrafish) for cycling normoxia-hypoxia imaging. Significantly, combined with a reliable preclinical genetically engineered murine model, such nanosensors successfully realize tracking of in vivo NSCLC lesions upon clear and gradient hypoxia signals without apparent long-term biotoxicity, illustrating their exciting potential for efficient NSCLC evaluation and prognosis.
为了探索高灵敏度和低毒性的技术来跟踪和评估非小细胞肺癌（NSCLC），这是世界上最致命的肿瘤之一，医生必须选择合适的治疗策略。为此，我们建议开发近红外（NIR）激发的纳米传感器，其中生物金属有机骨架（bio-MOF）基质内的供体和受体对被精确控制，以合理化上转换Förster 共振能量转移（FRET），从而用于检测肿瘤内部的 O 浓度，减少信号干扰和健康损害。在近红外激发下，所制备的核/卫星纳米传感器表现出更高的 FRET 效率和可逆的缺氧响应，具有高灵敏度，在体外和体内（斑马鱼）都可有效地进行循环正常氧-缺氧成像。重要的是，结合可靠的临床前基因工程小鼠模型，这种纳米传感器能够在没有明显长期生物毒性的情况下，实现对体内 NSCLC 病变的跟踪，并显示出清晰和梯度缺氧信号，这表明它们在高效 NSCLC 评估和预后方面具有令人兴奋的潜力。
