Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences Wolynska 35, 60-637 Poznan, Poland.
Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences Wolynska 35, 60-637 Poznan, Poland.
Biomed Pharmacother. 2020 May;125:110026. doi: 10.1016/j.biopha.2020.110026. Epub 2020 Feb 25.
Type 2 diabetes is associated with inflammatory and oxidative stress. Resveratrol, a naturally occurring diphenolic compound, was shown to improve glycemic control and alleviate metabolic disturbances in Goto-Kakizaki (GK) rats, a non-obese model of type 2 diabetes. However, in GK rats effects of resveratrol addressing inflammatory and oxidative stress were not explored. The present study aimed to determine anti-inflammatory and anti-oxidative properties of resveratrol in these rats. GK and Sprague-Dawley (SD) rats were divided into 4 groups: GK control, GK treated with resveratrol, SD control and SD treated with resveratrol. Resveratrol (20 mg/kg b.w.) was given once a day for 10 weeks. It was shown that contents of inflammatory markers, interleukin 6 (IL-6), interleukin 1 β (IL-1β), tumor necrosis factor α (TNF-α) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), were increased in the skeletal muscle of diabetic rats, but these effects were prevented by resveratrol therapy. Similarly, amounts of IL-1β and TNF-α were elevated in livers of GK rats; however, this rise was alleviated in resveratrol-treated animals. Moreover, the contents of inflammation-related factors (IL-6, IL-1β, TNF-α and NF-κB) were augmented in adipose tissue of GK rats; nevertheless, in this tissue resveratrol was ineffective. Resveratrol reduced also lipid peroxidation in the skeletal muscle, reduced activities of glutathione peroxidase in blood serum and catalase in the livers of GK rats. Our new findings show that resveratrol therapy results in relieving inflammatory and oxidative stress in GK rats, which may be largely associated with the alleviation of metabolic disturbances in this model of diabetes. Nevertheless, it was demonstrated that the efficacy of resveratrol action is tissue-specific.
2 型糖尿病与炎症和氧化应激有关。白藜芦醇是一种天然存在的二酚化合物，已被证明可改善 GK（Goto-Kakizaki）大鼠的血糖控制并减轻代谢紊乱，GK 大鼠是 2 型糖尿病的非肥胖模型。然而，在 GK 大鼠中，白藜芦醇对炎症和氧化应激的作用尚未得到探索。本研究旨在确定白藜芦醇在这些大鼠中的抗炎和抗氧化特性。将 GK 和 Sprague-Dawley（SD）大鼠分为 4 组：GK 对照组、GK 用白藜芦醇治疗组、SD 对照组和 SD 用白藜芦醇治疗组。白藜芦醇（20mg/kg b.w.）每天给药一次，共 10 周。结果表明，糖尿病大鼠骨骼肌中炎症标志物白细胞介素 6（IL-6）、白细胞介素 1β（IL-1β）、肿瘤坏死因子α（TNF-α）和核因子κB 轻链增强子的激活 B 细胞（NF-κB）的含量增加，但白藜芦醇治疗可预防这些作用。同样，GK 大鼠肝脏中 IL-1β 和 TNF-α 的含量也升高；然而，在白藜芦醇治疗的动物中，这种升高得到了缓解。此外，GK 大鼠脂肪组织中与炎症相关的因子（IL-6、IL-1β、TNF-α和 NF-κB）含量增加；然而，白藜芦醇在该组织中无效。白藜芦醇还降低了骨骼肌中的脂质过氧化，降低了 GK 大鼠血清中谷胱甘肽过氧化物酶的活性和肝脏中的过氧化氢酶的活性。我们的新发现表明，白藜芦醇治疗可缓解 GK 大鼠的炎症和氧化应激，这可能在很大程度上与该糖尿病模型代谢紊乱的缓解有关。然而，研究表明，白藜芦醇作用的疗效是组织特异性的。
