Min Jialing, Wang Zitong, Liang Chenglin, Li Wenjie, Shao Jie, Zhu Kunrui, Zhou Lu, Cheng Jing, Luo Shihong, Yu Lehan, Wu Yudong, Xie Mingyong, Hu Xiaojuan
Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Nanchang University, Nanchang 330006, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
J Agric Food Chem. 2020 Mar 18;68(11):3485-3494. doi: 10.1021/acs.jafc.9b08107. Epub 2020 Mar 9.
To date, there has been limited information on phytoestrogen (PE) exposure and metabolism in breastfed infants. In the present work, 50 sample pairs of Chinese breastfed infants' urine and the corresponding breast milk were collected. The contents of the relevant PE metabolites in the biosamples were detected via liquid chromatography-tandem mass spectrometry. The correlations between the PE metabolite contents in breastfed infants' urine and those in the corresponding breast milk were analyzed. The average concentrations of total PE metabolites in breast milk and urine were 0.27 and 0.23 nmol/mL, respectively. Genistein and enterolactone levels in the infant urine were positively correlated with their concentrations in the corresponding breast milk samples, which implies that urine excretion can be utilized as a noninvasive parameter for precise genistein and enterolactone intake assessment. Additionally, the efficiency of PE urine excretion showed significant differences across infants with different ages, genders, and durations of pregnancy.
迄今为止，关于母乳喂养婴儿体内植物雌激素（PE）暴露和代谢的信息有限。在本研究中，收集了50对中国母乳喂养婴儿的尿液及相应母乳样本。通过液相色谱 - 串联质谱法检测生物样本中相关PE代谢物的含量。分析了母乳喂养婴儿尿液和相应母乳中PE代谢物含量之间的相关性。母乳和尿液中总PE代谢物的平均浓度分别为0.27和0.23 nmol/mL。婴儿尿液中的染料木黄酮和肠内酯水平与相应母乳样本中的浓度呈正相关，这意味着尿液排泄可作为精确评估染料木黄酮和肠内酯摄入量的非侵入性参数。此外，不同年龄、性别和孕期的婴儿，其PE经尿液排泄的效率存在显著差异。
