Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne 50829, Germany.
Université Paris-Saclay, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, AgroParisTech, Institut Jean-Pierre Bourgin, Versailles 78000, France.
Proc Natl Acad Sci U S A. 2024 Jun 25;121(26):e2321877121. doi: 10.1073/pnas.2321877121. Epub 2024 Jun 21.
How tissue-level information encoded by fields of regulatory gene activity is translated into the patterns of cell polarity and growth that generate the diverse shapes of different species remains poorly understood. Here, we investigate this problem in the case of leaf shape differences between , which has simple leaves, and its relative that has complex leaves divided into leaflets. We show that patterned expression of the transcription factor CUP-SHAPED COTYLEDON1 in (ChCUC1) is a key determinant of leaf shape differences between the two species. Through inducible genetic perturbations, time-lapse imaging of growth, and computational modeling, we find that ChCUC1 provides instructive input into auxin-based leaf margin patterning. This input arises via transcriptional regulation of multiple auxin homeostasis components, including direct activation of WAG kinases that are known to regulate the polarity of PIN-FORMED auxin transporters. Thus, we have uncovered a mechanism that bridges biological scales by linking spatially distributed and species-specific transcription factor expression to cell-level polarity and growth, to shape diverse leaf forms.
组织层面上受调控基因活性场编码的信息如何转化为产生不同物种多样化形状的细胞极性和生长模式，目前仍知之甚少。在这里，我们研究了 和其近缘种 之间叶片形状差异的这个问题， 具有简单的叶片，而其近缘种 则具有分成小叶的复杂叶片。我们表明，转录因子 CUP-SHAPED COTYLEDON1 在 中的模式表达（ChCUC1）是两种物种之间叶片形状差异的关键决定因素。通过诱导遗传干扰、生长的延时成像和计算建模，我们发现 ChCUC1 为基于生长素的叶片边缘模式形成提供了指导输入。这种输入是通过多个生长素稳态成分的转录调控产生的，包括已知调节 PIN-FORMED 生长素转运蛋白极性的 WAG 激酶的直接激活。因此，我们发现了一种通过将空间分布和物种特异性转录因子表达与细胞水平的极性和生长联系起来从而形成多样化叶片形状的机制，从而连接了不同的生物学尺度。
