An Yang, Panayi Adriana C, Mi Bobin, Fu Siqi, Orgill Dennis P
Division of Plastic Surgery, Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Mass.
Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
Plast Reconstr Surg Glob Open. 2020 Jan 17;8(1):e2587. doi: 10.1097/GOX.0000000000002587. eCollection 2020 Jan.
Plastic surgeons desire more efficient methods of processing lipoaspirate when performing fat grafting procedures. We compared, in a preclinical study, the quantity and quality of lipoaspirate processed by a novel Poloxamer Wash, Absorption, mesh filtration System (PWAS) to a frequently used Ringer's Lactate wash, Decant, and mesh filtration System (RLDS).
Lipoaspirate from 10 patients was processed with the RLDS and PWAS systems. The processed lipoaspirate from each device was centrifuged to quantify the amount of fat, free oil, and aqueous components remaining in the fat graft. A trypan blue dye exclusion test assessed cell viability. The processing time for the lipoaspirate was also measured.
The 10-patient average fat volume processed and available for grafting was similar using both systems. The adipose volume fraction of PWAS was greater (89% ± 3%) than RLDS (76% ± 10%, = 0.02). The trypan blue exclusion values and processing time were similar for both systems. Oil was efficiently removed from the lipoaspirate, and both systems processed fat efficiently.
The PWAS effectively cleans lipoaspirate with increased fat concentration.
整形外科医生在进行脂肪移植手术时，希望有更高效的脂肪抽吸物处理方法。在一项临床前研究中，我们将一种新型泊洛沙姆洗涤、吸收、滤网过滤系统（PWAS）处理的脂肪抽吸物的数量和质量，与常用的乳酸林格氏液洗涤、倾析和滤网过滤系统（RLDS）进行了比较。
用RLDS和PWAS系统处理10名患者的脂肪抽吸物。将每个装置处理后的脂肪抽吸物离心，以量化脂肪移植物中剩余的脂肪、游离油和水性成分的量。台盼蓝染料排除试验评估细胞活力。还测量了脂肪抽吸物的处理时间。
使用两种系统处理的10名患者的平均可用于移植的脂肪体积相似。PWAS的脂肪体积分数（89%±3%）高于RLDS（76%±10%，P = 0.02）。两种系统的台盼蓝排除值和处理时间相似。脂肪抽吸物中的油被有效去除，两种系统都能有效地处理脂肪。
PWAS能有效清洁脂肪抽吸物并提高脂肪浓度。
