McIver V C, Tsang A S, Symonds N E, Perkins N R, Uquillas E, Dart C M, Jeffcott L B, Dart A J
Research and Clinical Training Unit, University Veterinary Teaching Hospital, The University of Sydney, Camden, New South Wales, Australia.
School of Veterinary Science, The University of Queensland, Gatton Campus, Gatton, Queensland, Australia.
Aust Vet J. 2020 Jun;98(6):250-255. doi: 10.1111/avj.12932. Epub 2020 Feb 24.
Evaluate the effect of topical 1% cannabidiol on second intention wound healing in distal limb wounds of horses.
Experimental.
Six Standardbred horses.
A total of five 2.5 cm × 2.5 cm full thickness skin wounds were created on the dorsomedial aspect of the metacarpi of 6 horses. Wounds were contaminated with faeces on the day of wound creation. Each wound was then assigned to a treatment group; compounded 1% cannabidiol in unique manuka factor (UMF) 5 manuka honey, UMF 5 manuka honey, UMF 20 manuka honey or saline. Each treatment was applied topically daily for a total of 42 days. Legs were bandaged and bandages were changed, daily, for 13 days postoperatively. Digital photographs of each wound were taken on day 1 then weekly for 6 weeks. Wound size, daily healing rate and total time to healing were recorded and compared statistically.
Irrespective of the treatment, wounds did not retract as expected in the first 7 days after wound creation. There was no difference in wound area, daily healing rate, days to complete healing between treatment groups.
This preliminary study failed to demonstrate any difference in wound healing variables between treatment groups in this model of second intention wound healing. This was unexpected due to the established effects of UMF 20 manuka honey on wound healing using the same model. This may be due to systemic effects of cannabidiol and study design. Further research into the use of cannabidiol in equine wounds is warranted.
评估局部应用1%大麻二酚对马远端肢体伤口二期愈合的影响。
实验性研究。
6匹标准赛马。
在6匹马的掌骨背内侧制造总共5个2.5厘米×2.5厘米的全层皮肤伤口。伤口在创建当天被粪便污染。然后将每个伤口分配到一个治疗组；分别为添加独特麦卢卡因子（UMF）5的麦卢卡蜂蜜中的1%大麻二酚复方制剂、UMF 5麦卢卡蜂蜜、UMF 20麦卢卡蜂蜜或生理盐水。每种治疗方法每天局部应用，共42天。术后13天腿部进行包扎，且每天更换绷带。在第1天拍摄每个伤口的数码照片，然后每周拍摄一次，持续6周。记录伤口大小、每日愈合率和总愈合时间，并进行统计学比较。
无论采用何种治疗方法，伤口在创建后的前7天均未如预期那样收缩。各治疗组之间在伤口面积、每日愈合率、完全愈合天数方面没有差异。
在这个二期愈合伤口模型中，这项初步研究未能证明各治疗组在伤口愈合变量上存在任何差异。鉴于使用相同模型时UMF 20麦卢卡蜂蜜对伤口愈合已有的效果，这一结果出乎意料。这可能是由于大麻二酚的全身效应和研究设计所致。有必要对大麻二酚在马伤口中的应用进行进一步研究。
