Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, the Netherlands.
Unilever R&D Vlaardingen, Olivier van Noortlaan 120, 3133 AT Vlaardingen, the Netherlands.
Food Chem. 2020 Jul 1;317:126389. doi: 10.1016/j.foodchem.2020.126389. Epub 2020 Feb 22.
Glyceollins are a class of antimicrobial prenylated pterocarpans produced in soybean seedlings upon fungus elicitation. Priming with reactive oxygen species (ROS) prior to elicitation with Rhizopus oligosporus/oryzae (R) was investigated for its potential to enhance glyceollin production. ROS-priming prior to R-elicitation (ROS + R) increased glyceollin production (8.6 ± 0.9 µmol/g dry weight (DW)) more than 4-fold compared to elicitation without priming (1.9 ± 0.4 µmol/g DW). Furthermore, ROS-priming was superior to two physical primers which were used as benchmark primers, namely slicing (5.0 ± 0.6 µmol glyceollins/g DW) and sonication (4.8 ± 1.0 µmol glyceollins/g DW). Subsequently, the robustness of ROS + R was assessed by applying it to another soybean cultivar, where it also resulted in a significantly higher glyceollin content than R-elicitation without priming. ROS-priming prior to elicitation provides opportunities for improving the yield in large-scale production of natural antimicrobials due to the ease of application and the robustness of the effect across cultivars.
大豆幼苗在真菌诱导下产生的一组抗菌类异戊烯基紫檀烷，称为 Glyceollins。本研究旨在探讨在 Rhizopus oligosporus/oryzae（R）诱导前用活性氧（ROS）进行预处理对提高 Glyceollin 产量的潜在作用。与未经预处理的 R 诱导相比（1.9 ± 0.4 μmol/g DW），ROS 预处理后再进行 R 诱导（ROS + R）使 Glyceollin 产量增加了 4 倍以上（8.6 ± 0.9 μmol/g DW）。此外，ROS 预处理优于两种物理引物（切片和超声），这两种物理引物作为基准引物，其 Glyceollin 产量分别为 5.0 ± 0.6 μmol/g DW 和 4.8 ± 1.0 μmol/g DW。随后，通过将 ROS + R 应用于另一种大豆品种来评估其稳健性，结果表明，ROS + R 也显著提高了未经预处理的 R 诱导的 Glyceollin 含量。由于易于应用且效果在不同品种间具有稳健性，因此在诱导前进行 ROS 预处理为提高天然抗菌剂的产量提供了机会。
