Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany.
Institute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, Germany.
Int J Environ Res Public Health. 2020 Feb 21;17(4):1412. doi: 10.3390/ijerph17041412.
Bovine brucellosis remains a persistent infection in ruminants in Pakistan. A total of 828 (409 buffaloes and 419 cattle) sera were collected from 11 institutional-owned livestock farms in Punjab, Pakistan. The samples were tested by rose bengal plate agglutination test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA). The seroprevalence along with 95% confidence interval (CI) was determined. Univariable and multivariable analysis of the epidemiological background data was conducted and odds ratio (OR) was calculated to understand any association between the risk factors and the seroprevalence. An overall seroprevalence of 3.9% (Positive/Tested = 32/828) and 3.3% (27/828) was detected by RBPT and iELISA, respectively. The seroprevalence of 5.6% (CI 3.6-8.3) and 4.7%, (CI 2.8-7.2) and the odds ratio of 2.63 (CI 1.20-5.77) and 2.50 (CI 1.08-5.78) for testing positive by RBPT and iELISA, respectively were significantly higher ( < 0.05) in buffaloes than in cattle. Breed, sex, history of abortion and retention of fetal membranes (RFM) in the animals were not found statistically significantly associated with the infection. RBPT and iELISA based results agreed almost perfect ( = 0.877). In total, -DNA (9/27) was amplified from seropositive samples by real-time polymerase chain reaction. This study identified for the first time the etiological agents of brucellosis at a molecular level at institutional-owned livestock farms in Pakistan.
巴基斯坦的牛布鲁氏菌病仍然是反刍动物中的持续感染。从巴基斯坦旁遮普省的 11 个机构所有的畜牧场采集了总共 828 份（409 份水牛血清和 419 份牛血清）血清样本。这些样本用虎红平板凝集试验（RBPT）和间接酶联免疫吸附试验（iELISA）进行了检测。确定了血清流行率及其 95%置信区间（CI）。对流行病学背景数据进行了单变量和多变量分析，并计算了优势比（OR），以了解危险因素与血清流行率之间的任何关联。通过 RBPT 和 iELISA 分别检测到总体血清流行率为 3.9%（阳性/检测=32/828）和 3.3%（27/828）。水牛的血清流行率为 5.6%（CI 3.6-8.3）和 4.7%（CI 2.8-7.2），而 RBPT 和 iELISA 检测阳性的优势比分别为 2.63（CI 1.20-5.77）和 2.50（CI 1.08-5.78），均显著高于牛（<0.05）。品种、性别、动物流产和胎膜滞留（RFM）史与感染无统计学显著关联。基于 RBPT 和 iELISA 的结果几乎完全一致（=0.877）。总共从血清阳性样本中通过实时聚合酶链反应扩增了- DNA（9/27）。本研究首次在巴基斯坦的机构所有畜牧场从分子水平上确定了布鲁氏菌病的病原体。
