使用无菌动物模型研究肠道微生物群在研究中的影响：逐步的无菌设置与管理
Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management.
作者信息
Gabay Odile, Vicenty Jonathan, Smith Dylan, Tiffany Linda, Ascher Jill, Curry Tina, Dennis John, Clouse Kathleen A
机构信息
U.S. Food and Drug Administration, Center for Drug Evaluation and Research, Office of Biotechnology Products, Division of Biotechnology Review and Research 1, Silver Spring, MD 20993, USA.
U.S. Food and Drug Administration, Center for Biologics Evaluation and Research, Office of Management, Division of Veterinary Services, Silver Spring, MD 20993, USA.
出版信息
Methods Protoc. 2020 Feb 22;3(1):18. doi: 10.3390/mps3010018.
The particularly unique composition of the gut microbiota has the potential to influence the health or disease status of animal and human hosts. Altering the homeostasis of the host-bacteria could lead to changes in gut flora that result in disease or activation of a specific immunological response, which could explain the variations observed in patient responses to current therapies. A standardized model is crucial for studying the influence of the gut microbiota on therapeutic modalities. A step by step mouse model and sterility management system that compares a control strain of C57BL/6 mice to the established C57BL/6 germ-free (GF) strain has been developed. The GF BL/6 mouse phenotype is well established, and the anatomical differences between the GF and control mice were evident in this model. This method could be applied to research studies investigating the microbiome impact, the response to various therapies, or disease transfer via fecal transplants. A standardized sterility maintenance method is crucial in this context.
肠道微生物群独特的组成有可能影响动物和人类宿主的健康或疾病状态。改变宿主与细菌之间的平衡可能会导致肠道菌群变化，进而引发疾病或激活特定的免疫反应，这或许可以解释患者对当前治疗的反应差异。标准化模型对于研究肠道微生物群对治疗方式的影响至关重要。现已开发出一种逐步建立的小鼠模型和无菌管理系统，该系统将C57BL/6小鼠的对照菌株与已确立的C57BL/6无菌（GF）菌株进行比较。GF BL/6小鼠的表型已得到充分确立，在该模型中，GF小鼠与对照小鼠之间的解剖学差异很明显。此方法可应用于研究微生物组影响、对各种治疗的反应或通过粪便移植进行疾病转移的研究。在这种情况下，标准化的无菌维持方法至关重要。
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