Tefera Mulugeta Mihrete, Altaye Birhanetensay Masresha, Yimer Ebrahim M, Berhe Derbew Fikadu, Tadesse Bekele Senait
Department of Pharmacy, Bahirdar Health Science College, Bahirdar, Ethiopa.
Department of Pharmacology and Toxicology, College of Health Sciences, Mekelle University, Mekelle, Ethiopia.
J Exp Pharmacol. 2020 Jan 31;12:39-45. doi: 10.2147/JEP.S228834. eCollection 2020.
seed has been used in traditional practices to treat various ailments, including diabetes mellitus, in Ethiopia. Previous phytochemical screening studies indicated that germination of the seed of contains more bioactive constituents compared to raw seeds. The aim of this study was to investigate the antidiabetic activity of an aqueous methanol extract of germinated seed extract in streptozotocin (Stz)-induced diabetic mice.
The antidiabetic effect of germinated seed extract was determined using Stz-induced diabetic mice. An 80% aqueous methanol extract of germinated seed at doses of 100, 200, and 400 mg/kg was used in the treatment group. Glibenclamid (5 mg/kg) and dimethyl sulfoxide 2% were used as positive and negative controls, respectively. The test extract and controls were given daily for 3 weeks. Blood-glucose levels and body-weight changes were measured weekly. Lipid-profile levels were measured at the end of each experiment. Oral glucose-tolerance tests were performed to evaluate the postprandial effect of the extract.
The aqueous methanol extract of germinated significantly reduced blood-glucose levels and increased body weight (<0.05). The extract also improved serum-lipid profiles in diabetic mice after 21 days (<0.05). The seed extract also resulted in significant reductions in blood-glucose levels after an oral glucose load in normal mice (<0.05).
An aqueous methanol extract of germinated seed has both antidiabetic and antihyperlipidemic effects.
在埃塞俄比亚，[种子名称]种子已被用于传统医学治疗包括糖尿病在内的各种疾病。先前的植物化学筛选研究表明，[种子名称]种子发芽后比未发芽种子含有更多的生物活性成分。本研究旨在探讨发芽的[种子名称]种子水甲醇提取物对链脲佐菌素（Stz）诱导的糖尿病小鼠的抗糖尿病活性。
使用Stz诱导的糖尿病小鼠来测定发芽的[种子名称]种子提取物的抗糖尿病作用。治疗组使用剂量为100、200和400mg/kg的发芽的[种子名称]种子80%水甲醇提取物。格列本脲（5mg/kg）和2%二甲亚砜分别用作阳性和阴性对照。试验提取物和对照每天给药，持续3周。每周测量血糖水平和体重变化。在每个实验结束时测量血脂水平。进行口服葡萄糖耐量试验以评估提取物的餐后效应。
发芽的[种子名称]种子水甲醇提取物显著降低血糖水平并增加体重（P<0.05）。21天后，该提取物还改善了糖尿病小鼠的血清脂质谱（P<0.05）。在正常小鼠口服葡萄糖负荷后，该种子提取物也导致血糖水平显著降低（P<0.05）。
发芽的[种子名称]种子水甲醇提取物具有抗糖尿病和抗高血脂作用。
