Cherie Melaku Bamlaku, Amare Gedefaw Getnet
Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.
J Exp Pharmacol. 2020 Jun 22;12:181-189. doi: 10.2147/JEP.S258522. eCollection 2020.
Nature has gifted a variety of phytochemicals having a potential effect against diabetes mellitus. has been used as a remedy for the treatment of diabetes mellitus. The study aimed to determine the in vivo antidiabetic potential of hydromethanolic seed extract of the plant.
Dried seeds of were macerated in hydromethanol. Three doses (100, 200, and 400 mg/kg) of the seed extract were given orally to normoglycemic, glucose-loaded, and Streptozocin-induced diabetic mice. Diphenyl-1-picrylhydrazine (DPPH) assay was employed to determine antioxidant activity of the seed extract.
All doses of hydromethanolic seed extract of were devoid of any significant hypoglycemic effect in normoglycemic mice compared to the negative control group. Acute glucose reduction was significant (<0.05 at 100, <0.01 at 200 and 400 mg/kg) with respect to negative control in oral glucose-loaded mice. All doses of seed extract significantly (<0.0l) reduced blood glucose level on weeks 1 and 2 in STZ-induced daily-treated diabetic mice. The seed extract at the doses of 200 and 400 mg/kg significantly (<0.05) improved the body weight of diabetic mice on weeks 1 and 2. A low (100 mg/kg) dose of the seed extract delayed and significantly (<0.05) increased body weight of mice on week 2 compared to negative control. The finding showed that the antioxidant activity of the hydromethanolic seed extract was concentration dependent and comparable with ascorbic acid. IC50 of the seed extract and ascorbic acid was found to be 11.95 and 5.07 mg/mL, respectively.
The findings of the study showed that hydromethanolic seed extract of endowed significant antihyperglycemic and antioxidant activity.
大自然赋予了多种具有抗糖尿病潜在作用的植物化学物质。[植物名称]已被用作治疗糖尿病的药物。本研究旨在确定该植物水甲醇种子提取物的体内抗糖尿病潜力。
将[植物名称]的干燥种子在水甲醇中浸渍。给正常血糖、葡萄糖负荷和链脲佐菌素诱导的糖尿病小鼠口服三种剂量（100、200和400毫克/千克）的种子提取物。采用二苯基-1-苦基肼（DPPH）法测定种子提取物的抗氧化活性。
与阴性对照组相比，[植物名称]水甲醇种子提取物的所有剂量在正常血糖小鼠中均无明显的降血糖作用。在口服葡萄糖负荷小鼠中，相对于阴性对照，急性血糖降低显著（100毫克/千克时<0.05，200和400毫克/千克时<0.01）。在链脲佐菌素诱导的每日治疗糖尿病小鼠中，所有剂量的种子提取物在第1周和第2周均显著（<0.01）降低血糖水平。200和400毫克/千克剂量的种子提取物在第1周和第2周显著（<0.05）改善了糖尿病小鼠的体重。与阴性对照相比，低剂量（100毫克/千克）的种子提取物在第2周延迟并显著（<0.05）增加了小鼠体重。结果表明，水甲醇种子提取物的抗氧化活性呈浓度依赖性，且与抗坏血酸相当。种子提取物和抗坏血酸的IC50分别为11.95和5.07毫克/毫升。
该研究结果表明，[植物名称]水甲醇种子提取物具有显著的降血糖和抗氧化活性。
