Walker Brett, Amato Chad, Palyvoda Olena, Vangipuram Sharada, Weaver Martin, Sayeed Zain, Talha Padela Muhammad, Yassir Walid K
Department of Orthopedics, DMC Children's Hospital of Michigan, Detroit, MI 48201, USA.
Department of Chemistry, Wayne State University, Detroit, MI 48201, USA.
Int J Pediatr. 2020 Jan 21;2020:4717385. doi: 10.1155/2020/4717385. eCollection 2020.
Surgical site infection is a relatively common and devastating complication following pediatric orthopedic surgery. Many infections have been determined to be the result of settled airborne particles on surgical equipment and the sterile field. Fiberglass casts are commonly used orthopedic fixation devices before and after surgery; however, fiberglass casting material is expelled during the removal process and represents an uninvestigated area for the possibility of cast saw dust as a source of airborne bacterial contamination in an operating room setting. This study evaluates the prevalence and distribution of microbiota on 90 pediatric casts by collecting and culturing fiberglass cast material from 90 pediatric casts. Bacterial identification was performed using a Bruker Biotyper Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry device. 81 out of 90 casts (90%) showed evidence of microbial contamination. Isolated species were very diverse and ranged from normal skin flora to opportunistic pathogens. The 5 most commonly isolated organisms were and Further investigation is required to determine if casting material is truly a cause of surgical site infection.
手术部位感染是小儿骨科手术后一种相对常见且具有破坏性的并发症。许多感染已被确定是手术设备和无菌区域上沉降的空气传播颗粒所致。玻璃纤维石膏是手术前后常用的骨科固定装置；然而，在拆除过程中玻璃纤维铸造材料会被排出，这代表了一个未被研究的领域，即在手术室环境中，石膏锯屑作为空气传播细菌污染源的可能性。本研究通过收集和培养90个小儿石膏的玻璃纤维铸造材料，评估了90个小儿石膏上微生物群的流行情况和分布。使用布鲁克生物分型基质辅助激光解吸/电离飞行时间质谱仪进行细菌鉴定。90个石膏中有81个（90%）显示出微生物污染的迹象。分离出的物种非常多样，从正常皮肤菌群到机会性病原体不等。最常分离出的5种微生物是……需要进一步调查以确定铸造材料是否真的是手术部位感染的原因。
