介绍肿瘤坏死因子在乳糜泻和1型糖尿病中作为一个重要因素。

Introducing tumor necrosis factor as a prominent player in celiac disease and type 1 diabetes mellitus.

作者信息

Rezaei-Tavirani Sina, Rostami-Nejad Mohammad, Vafaee Reza, Khalkhal Ensieh, Keramatinia Aliasghar, Ehsani-Ardakani Mohammad Javad, Razzaghi Mohammadreza

机构信息

Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Gastroenterol Hepatol Bed Bench. 2019;12(Suppl1):S123-S129.

PMID:32099612

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7011075/

Abstract

AIM

This study aimed to screen the common genes between celiac disease (CD) and type 1 diabetes mellitus to find critical ones.

BACKGROUND

Celiac disease is a chronic autoimmune disorder which is correlated to type 1 diabetes mellitus (T1DM) in several molecular pathways. Understanding the clear common molecular mechanism of both diseases is of interest to scientists.

METHODS

The related genes to the CD and T1DM were obtained from disease query of STRING and included in two separated PPI networks by Cytoscape software version 3.7.1. The networks were analyzed by network analyzer and the hub nodes were determined. The common hubs between the two networks were selected for further analysis and enriched via gene ontology using ClueGO plugin of Cytoscape software. Also, an action map was provided by Cluepedia application of Cytoscape software.

RESULTS

Two separated networks of 2000 and 430 genes were constructed related to T1DM and CD, respectively. A total of 84 and 28 hubs were determined for T1DM and CD, respectively. There were 11 common hubs between the two networks. The first top hubs of Type 1 Diabetes Mellitus and CD networks were insulin (INS) and tumor necrosis factor (TNF), respectively. Also, 77 biological terms and pathways (in five clusters) were related to the common hubs. Action map revealed a close relationship between hubs.

CONCLUSION

The result of this study indicated that TNF is key mediator of immune reactions in celiac disease and type 1 diabetes mellitus.

摘要

目的

本研究旨在筛选乳糜泻（CD）和1型糖尿病之间的共同基因，以找出关键基因。

背景

乳糜泻是一种慢性自身免疫性疾病，在多个分子途径中与1型糖尿病（T1DM）相关。了解这两种疾病明确的共同分子机制是科学家们感兴趣的。

方法

从STRING的疾病查询中获取与CD和T1DM相关的基因，并通过Cytoscape软件3.7.1版本纳入两个独立的蛋白质-蛋白质相互作用（PPI）网络。使用网络分析器对网络进行分析，并确定中心节点。选择两个网络之间的共同中心节点进行进一步分析，并使用Cytoscape软件的ClueGO插件通过基因本体进行富集。此外，通过Cytoscape软件的Cluepedia应用程序提供作用图谱。

结果

分别构建了与T1DM和CD相关的两个独立网络，包含2000个和430个基因。分别为T1DM和CD确定了总共84个和28个中心节点。两个网络之间有11个共同的中心节点。1型糖尿病和CD网络的前顶级中心节点分别是胰岛素（INS）和肿瘤坏死因子（TNF）。此外，77个生物学术语和途径（分为五个簇）与共同的中心节点相关。作用图谱显示中心节点之间存在密切关系。