Park Sooyeon, Jung Yong-Taek, Choi Su Jung, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2964-2969. doi: 10.1099/ijsem.0.002055. Epub 2017 Aug 18.
A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated JSSK-14T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain JSSK-14T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain JSSK-14T fell within the clade comprising the type strains of species of the genus Erythrobacter. Strain JSSK-14T exhibited 16S rRNA gene sequence similarity values of 97.09-98.42 % to the type strains of Erythrobactergangjinensis, Erythrobacteratlanticus and Erythrobacterluteus and of 94.31-96.97 % to the type strains of the other species of the genus Erythrobacter. Strain JSSK-14T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain JSSK-14T were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C content of strain JSSK-14T was 63.5 mol%, and its mean DNA-DNA relatedness values with the type strains of Erythrobactergangjinensis, Erythrobacteratlanticus and Erythrobacterluteus were 13-25 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain JSSK-14T is separated from recognized species of the genus Erythrobacter. On the basis of the data presented, strain JSSK-14T is considered to represent a novel species of the genus Erythrobacter, for which the name Erythrobacter aquimixticola sp. nov. is proposed. The type strain is JSSK-14T (=KCTC 52764T=NBRC 112765T).
从韩国济州岛海洋与淡水泉交汇处分离出一株革兰氏阴性、需氧、不运动、呈球状、卵形或杆状的细菌菌株，命名为JSSK-14T。菌株JSSK-14T在pH 7.0 - 8.0、30 °C以及1.0 - 2.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株JSSK-14T属于包含红杆菌属各物种模式菌株的进化分支。菌株JSSK-14T与江陵红杆菌、大西洋红杆菌和黄色红杆菌的模式菌株的16S rRNA基因序列相似性值为97.09 - 98.42 %，与红杆菌属其他物种的模式菌株的相似性值为94.31 - 96.97 %。菌株JSSK-14T以Q-10作为主要泛醌，以C18 : 1ω7c作为主要脂肪酸。菌株JSSK-14T的主要极性脂类为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和鞘糖脂。菌株JSSK-14T的DNA G+C含量为63.5 mol%，其与江陵红杆菌、大西洋红杆菌和黄色红杆菌模式菌株的平均DNA - DNA相关性值为13 - 25 %。不同的表型特征，以及系统发育和遗传独特性，表明菌株JSSK-14T与红杆菌属已确认的物种不同。根据所提供的数据，菌株JSSK-14T被认为代表红杆菌属的一个新物种，为此提出新名称混水红杆菌（Erythrobacter aquimixticola sp. nov.）。模式菌株为JSSK-14T（=KCTC 52764T=NBRC 112765T）。
