Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, Institute of Environment and Health, Jianghan University, Wuhan, 430056, China.
Department of Laboratory Medicine, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, 430016, China.
Analyst. 2020 Mar 16;145(6):2184-2190. doi: 10.1039/d0an00035c.
The continuing hurdle of developing foodborne pathogen detection techniques is that compromises must be made among simplicity, portability, speed, sensitivity, and quantitation. Herein, we fabricated quantum dot nanobeads (QDNS) by a layer-by-layer assembly of quantum dots on the surface of polymer nanospheres. QDNS exhibited higher fluorescence intensity than the quantum dots at the same particle number. Based on the quantum dot nanobeads as the signal reporter, a quantitative lateral flow immunoassay was demonstrated for Salmonella typhimurium detection with improved sensitivity, specificity and accuracy. A visual detection limit of 5 × 103 CFU mL-1Salmonella typhimurium within 10 min has been proved and demonstrated. Additionally, higher concentrations of non-Salmonella typhimurium bacteria have negligible effects on the detection of Salmonella typhimurium. The results of 50 single blind tests by 10 testers suggested that the assay exhibited 100% accuracy. The results illustrate that the assay provides a balance among simplicity, speed, sensitivity and accuracy, and it can be a favorable alternative for Salmonella typhimurium screening in various samples.
开发食源性病原体检测技术的持续障碍在于，在简单性、便携性、速度、灵敏度和定量之间必须做出折衷。在此，我们通过在聚合物纳米球表面上层层组装量子点来制造量子点纳米珠 (QDNS)。与相同数量的量子点相比，QDNS 表现出更高的荧光强度。基于量子点纳米珠作为信号报告器，建立了一种用于检测鼠伤寒沙门氏菌的定量侧向流动免疫分析方法，具有更高的灵敏度、特异性和准确性。已证明并证明了在 10 分钟内可以检测到 5×103 CFU mL-1 的鼠伤寒沙门氏菌的目视检测限。此外，较高浓度的非鼠伤寒沙门氏菌细菌对鼠伤寒沙门氏菌的检测几乎没有影响。由 10 名测试人员进行的 50 次单盲测试的结果表明，该测定方法的准确率为 100%。结果表明，该测定方法在简单性、速度、灵敏度和准确性之间取得了平衡，可作为各种样品中鼠伤寒沙门氏菌筛选的有利替代方法。
