Elorza M V, Villanueva J R, Sentandreu R
Biochim Biophys Acta. 1977 Mar 2;475(1):103-12. doi: 10.1016/0005-2787(77)90344-6.
Saccharomyces cerevisiae -136ts synthesized invertase in media containing maltose and sucrose. In the presence of glucose synthesis of enzyme took place when the sugar concentration was lower than 1%. At higher concentrations enzyme formation was repressed. Analysis of the glucose effect before RNA inhibition showed that the hexose interfered with the transcription of DNA into invertase messenger RNA. Translation of invertase messenger already formed was also inhibited and the kinetics of this effect was similar to that produced by cycloheximide. Invertase activity was independent of glucose suggesting that the hexose produces no catabolite inhibition of invertase activity. Inhibition of invertase translation by glucose turned out to be reversible but the amount of enzyme produced was dependent on duration of treatment. It is suggested that the catabolite repression of invertase synthesis produced by glucose operates at the levels of transcription and translation and produces an increase in the rate of mRNA degradation. The catabolite repression has no effect on secretion and does not interfere with the catalytic activity of invertase.
酿酒酵母-136ts在含有麦芽糖和蔗糖的培养基中合成转化酶。在葡萄糖存在的情况下，当糖浓度低于1%时酶开始合成。在较高浓度下，酶的形成受到抑制。在RNA抑制之前对葡萄糖效应的分析表明，己糖干扰了DNA转录为转化酶信使RNA的过程。已形成的转化酶信使的翻译也受到抑制，这种效应的动力学与环己酰亚胺产生的相似。转化酶活性与葡萄糖无关，这表明己糖对转化酶活性没有分解代谢抑制作用。葡萄糖对转化酶翻译的抑制结果是可逆的，但产生的酶量取决于处理时间。有人认为，葡萄糖产生的转化酶合成的分解代谢阻遏作用发生在转录和翻译水平，并导致mRNA降解速率增加。分解代谢阻遏对分泌没有影响，也不干扰转化酶的催化活性。
