CellNanOs - Center for Cellular Nanoanalytics Osnabrück, School of Biology/Chemistry, University of Osnabrück, Osnabrück, Germany.
Division of Microbiology, School of Biology/Chemistry, University of Osnabrück, Osnabrück, Germany.
Mol Cell Proteomics. 2020 May;19(5):900-912. doi: 10.1074/mcp.RA119.001841. Epub 2020 Feb 26.
Systemic infection and proliferation of intracellular pathogens require the biogenesis of a growth-stimulating compartment. The gastrointestinal pathogen commonly forms highly dynamic and extensive tubular membrane compartments built from -modified membranes (SMMs) in diverse host cells. Although the general mechanism involved in the formation of replication-permissive compartments of is well researched, much less is known regarding specific adaptations to different host cell types. Using an affinity-based proteome approach, we explored the composition of SMMs in murine macrophages. The systematic characterization provides a broader landscape of host players to the maturation of -containing compartments and reveals core host elements targeted by in macrophages as well as epithelial cells. However, we also identified subtle host specific adaptations. Some of these observations, such as the differential involvement of the COPII system, Rab GTPases 2A, 8B, 11 and ER transport proteins Sec61 and Sec22B may explain cell line-dependent variations in the pathophysiology of infections. In summary, our system-wide approach demonstrates a hitherto underappreciated impact of the host cell type in the formation of intracellular compartments by .
细胞内病原体的系统性感染和增殖需要刺激生长的隔间的生物发生。胃肠道病原体 通常在各种宿主细胞中形成高度动态和广泛的管状膜隔间，这些隔间由 - 修饰的膜 (SMM) 构建。尽管已经很好地研究了 形成复制允许隔间的一般机制，但对于不同宿主细胞类型的具体适应知之甚少。我们使用基于亲和性的蛋白质组学方法探索了鼠巨噬细胞中 SMM 的组成。系统的特征描述提供了更广泛的宿主参与者景观，以成熟的 - 包含隔间，并揭示了 巨噬细胞和上皮细胞中的核心宿主元素。然而，我们也发现了微妙的宿主特异性适应。其中一些观察结果，例如 COPII 系统、Rab GTPases 2A、8B、11 和内质网转运蛋白 Sec61 和 Sec22B 的差异参与，可能解释了 感染中细胞系依赖性的病理生理学变化。总之，我们的系统范围方法表明，宿主细胞类型在 形成细胞内隔间方面具有迄今为止被低估的影响。
