iRIP unit, Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium.
VIB-UGent Center for Medical Biotechnology, Ghent, Belgium.
PLoS Pathog. 2023 Aug 3;19(8):e1011183. doi: 10.1371/journal.ppat.1011183. eCollection 2023 Aug.
By applying dual proteome profiling to Salmonella enterica serovar Typhimurium (S. Typhimurium) encounters with its epithelial host (here, S. Typhimurium infected human HeLa cells), a detailed interdependent and holistic proteomic perspective on host-pathogen interactions over the time course of infection was obtained. Data-independent acquisition (DIA)-based proteomics was found to outperform data-dependent acquisition (DDA) workflows, especially in identifying the downregulated bacterial proteome response during infection progression by permitting quantification of low abundant bacterial proteins at early times of infection when bacterial infection load is low. S. Typhimurium invasion and replication specific proteomic signatures in epithelial cells revealed interdependent host/pathogen specific responses besides pointing to putative novel infection markers and signalling responses, including regulated host proteins associated with Salmonella-modified membranes.
通过对沙门氏菌肠炎亚种（鼠伤寒沙门氏菌）与上皮宿主（这里是感染人类宫颈癌细胞的鼠伤寒沙门氏菌）相互作用的双蛋白质组分析，我们获得了一个关于感染过程中宿主-病原体相互作用的详细的、相互依存的、整体的蛋白质组学视角。基于数据独立采集（DIA）的蛋白质组学被发现优于基于数据依赖采集（DDA）的工作流程，尤其是在鉴定感染过程中细菌蛋白质组反应下调方面，因为它允许在细菌感染负荷较低的早期感染时间点对低丰度的细菌蛋白质进行定量分析。上皮细胞中沙门氏菌入侵和复制的特异性蛋白质组学特征不仅揭示了宿主/病原体之间相互依存的特异性反应，还指出了潜在的新感染标志物和信号反应，包括与沙门氏菌修饰的膜相关的受调控的宿主蛋白。
