Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
Pest Manag Sci. 2020 May;76(5):1612-1617. doi: 10.1002/ps.5804. Epub 2020 Mar 11.
Vip3A proteins are widely used for controlling pest Lepidoptera. Different binding sites with different receptors in the insect midgut membrane and lack of cross-resistance with crystal (Cry) proteins enhance their applicability, as both single proteins and proteins pyramided with Cry proteins in transgenic Bt crops. Vip3A proteins are effective but there is relatively little information about their structure, function, activation, specificity, and mode of action. In addition, the mechanism of insect resistance to these proteins is unknown. Phylogenetic analysis and multiple sequence alignment showed that Vip3A proteins are genetically distant from Cry proteins. The mode of action and insecticidal activity of Vip3A proteins are discussed in this review. This review also provides detailed information about the Vip3A protein family that may aid in the design of more efficient pest management strategies in response to insect resistance to insecticidal proteins. © 2020 Society of Chemical Industry.
Vip3A 蛋白被广泛用于防治鳞翅目害虫。不同的结合位点与昆虫中肠膜上的不同受体结合，并且与 Cry 蛋白缺乏交叉抗性，这增强了它们的适用性，因为它们既可以作为单一蛋白，也可以与转 Bt 作物中的 Cry 蛋白共表达。Vip3A 蛋白是有效的，但关于它们的结构、功能、激活、特异性和作用模式的信息相对较少。此外，昆虫对这些蛋白产生抗性的机制尚不清楚。系统发育分析和多重序列比对表明，Vip3A 蛋白在遗传上与 Cry 蛋白相距甚远。本文讨论了 Vip3A 蛋白的作用模式和杀虫活性。本综述还提供了有关 Vip3A 蛋白家族的详细信息，这可能有助于设计更有效的害虫管理策略，以应对昆虫对杀虫蛋白的抗性。© 2020 英国化学学会。
