Chinatangkul Nawinda, Limmatvapirat Chutima, Nunthanid Jurairat, Luangtana-Anan Manee, Sriamornsak Pornsak, Limmatvapirat Sontaya
Faculty of Pharmacy, Siam University, Bangkok 10160, Thailand.
Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
Asian J Pharm Sci. 2018 Sep;13(5):459-471. doi: 10.1016/j.ajps.2017.12.006. Epub 2017 Dec 27.
The aim of this study was to elucidate the optimized fabrication factors influencing the formation and properties of shellac (SHL) nanofibers loaded with an antimicrobial monolaurin (ML). The main and interaction effects of formulation and process parameters including SHL content (35%-40% w/w), ML content (1%-3% w/w), applied voltage (9-27 kV) and flow rate (0.4-1.2 ml/h) on the characteristic of nanofibers were investigated through a total of 19 experiments based on a full factorial design with three replicated center points. As a result, the SHL content was the major parameter affecting fiber diameter. Another response result revealed that the SHL content would be also the most significant negative impact on amount of beads. An increase in the concentration of SHL leaded to a reduction in the amount of beads. From the results of characterization study, it was proved that ML might be entrapped between the chains of SHL during the electrospinning process exhibiting an excellent encapsulation. According to the response surface area, small (488 nm) and beadless (0.48) fibers were obtained with the SHL and ML contents of 37.5% and 1.1% w/w respectively, at the applied voltage of 18 kV and the flow rate of 0.8 ml/h. In addition, the results of the kill-kinetic studies showed that SHL nanofibers loaded with ML exhibited an excellent antibacterial activity against , while was less affected due to the hydrophilic structure of the its outer membrane. ML also exerted an antifungal activity by reducing the number of colonies. Based on their structural and antimicrobial properties, SHL nanofibers containing ML could be potentially used as a medicated dressing for wound treatment.
本研究的目的是阐明影响负载抗菌月桂酸单甘油酯（ML）的紫胶（SHL）纳米纤维形成及性能的优化制备因素。通过基于全因子设计并带有三个重复中心点的总共19次实验，研究了包括SHL含量（35%-40% w/w）、ML含量（1%-3% w/w）、施加电压（9-27 kV）和流速（0.4-1.2 ml/h）在内的配方和工艺参数对纳米纤维特性的主要和交互作用。结果表明，SHL含量是影响纤维直径的主要参数。另一个响应结果显示，SHL含量对珠粒数量也有最显著的负面影响。SHL浓度的增加导致珠粒数量减少。从表征研究结果可知，在静电纺丝过程中，ML可能被困在SHL链之间，表现出优异的包封效果。根据响应表面积，在施加电压为18 kV和流速为0.8 ml/h时，分别使用37.5%和1.1% w/w的SHL和ML含量可获得小直径（约488 nm）且无珠粒（约0.48）的纤维。此外，杀菌动力学研究结果表明，负载ML的SHL纳米纤维对[具体细菌名称未给出]表现出优异的抗菌活性，而[具体细菌名称未给出]由于其外膜的亲水性结构受影响较小。ML还通过减少[具体真菌名称未给出]菌落数量发挥抗真菌活性。基于其结构和抗菌性能，含ML的SHL纳米纤维有潜力用作伤口治疗的药用敷料。
